objective(s):n-myc downstream regulated gene 2 (ndrg2) is a candidate gene for tumor suppression. the expression of ndrg2 is down-regulated in several tumors including lung cancer. the aim of this study was to explore the effect of ndrg2 overexpression on invasion, migration, and enzymatic activity of matrix metalloproteinase-2 (mmp-2) and -9 (mmp-9) in human lung adenocarcinoma a549 cells.  ma...

The gluten-free pasta (GFP) has a higher cost and is often brittle, pale, poorly balanced in macro micronutrients when compared to wheat-based pasta. This study aimed use the biofortified sweet potato flour, natural source of color provitamin A ingredient, with rice hydrolyzed soy protein concentrate, carboxymethyl cellulose gum produce GFP, using central composite rotatable design three factor...

this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

La Proteína Verde Fluorescente (Green Fluorescent Protein, GFP) es ampliamente utilizada en ensayos in vivo e vitro. Se han generado múltiples variantes de esta proteína para diversificar sus características, como la GFP-enhancer (EGFP) que emite una señal fluorescencia 35 veces mayor comparación con silvestre, siendo implementada fusión estudios localización y estabilidad estructural, entre ot...

Effluents from wastewater treatment works (WwTW) exhibit both temporal and spatial variation in oestrogenicity, however few studies have attempted to quantify how this affects biological responses fish. Here we used an oestrogen-responsive green fluorescent protein (ERE-GFP) transgenic zebrafish (Danio rerio) oestrogenic activity health effects for exposure three different WwTW effluents. Endpo...

Objective(s): Neural stem/progenitor cells (NS/PCs) hold a great potential for delivery of therapeutic agents into the injured regions of the brain. Efficient gene delivery using NS/PCs may correct a genetic defect, produce therapeutic proteins or neurotransmitters, and modulate enzyme activation. Here, we investigated the efficiency of a recombinant lentivirus vector ...

objective(s) mesenchymal stem cells (mscs) are nonhematopoietic stromal cells that are capable of differentiating into and contribute to the regeneration of mesenchymal tissues. human mesenchymal stem cells (hmscs) are ideal targets in cell transplantation and tissue engineering. enhanced green fluorescent protein (egfp) has been an important reporter gene for gene therapy. the aim of this stud...