An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5µm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mobi...

Ionic liquids, considered "green" chemicals, are widely used in many areas of analytical chemistry due to their unique properties. Recently, ionic liquids have been used as a kind of novel additive in separation and combined with silica to synthesize new stationary phase as separation media. This review will focus on the properties and mechanisms of ionic liquids and their potential application...

Abstract Nowadays, when analysts develop a new method, they are taking into consideration the green aspects of developed method. Two chromatographic methods were for determination antidepressant pharmaceutical combination nortriptyline hydrochloride and fluphenazine in presence their potential impurities assessed using four tools; eco-scale, analytical greenness profile (AGP), metric approach (...

RP-HPLC has largely been the analytical method of choice in the pharmaceutical industry for many years because of the poor aqueous solubility and hydrophobicity of most small molecule drug candidates. RP-HPLC coupled to MS has provided an excellent analytical tool for qualitatively and quantitatively determining levels of drug molecules or drug metabolites for studies such as purity assessment ...

Validasi metode analisis adalah suatu proses dalam menjamin akurasi analisis, reprodusibel, spesifik dan stabil dari kisaran sesuai dengan tujuan penggunaan. mencakup semua prosedur yang menunjukkan pengukuran kuantitatif analit matriks biologis seperti plasma. Penentuan konsentrasi obat plasma merupakan hal kompleks karena kompleks. Metformin agen antihiperglikemik oral digunakan pengelolaan d...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...