A pair of degenerate primers, GMPF1 and GMPR1, was designed on the basis of alignment of previously reported Grapevine fanleaf virus (GFLV) movement protein (MP) nucleotide sequences from Iran and other parts of the world. cDNA was synthesized by the use of Oligo d(T)18 from total RNA extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (PCR) with the d...

The research of sanitary status and virus presence was conducted on three native Croatian grapevine varieties (Kujudžuša bijela, Zlatarica vrgorska Trnjak crni) that are grown in Dalmatia region. investigation done 492 vines from 31 vineyards. Vines were tested by ELISA (Enzyme-Linked Immunosorbent Assay) four main economic viruses: arabis mosaic (ArMV), fanleaf (GFLV), leafroll-associated 1 3 ...

The RNA-dependent RNA polymerase (1E Pol ) is involved in replication of grapevine fanleaf virus (GFLV, Nepovirus , Secoviridae and causes vein clearing symptoms Nicotiana benthamiana . Information on protein 1E interaction with other viral host proteins scarce. To study biology, three GFLV infectious clones, i.e. GHu (a symptomatic wild-type strain), GHu-1E K802G (an asymptomatic mutant) F13 w...

Grapevine virus G (GVG) and grapevine H (GVH) (genus Vitivirus) are recently discovered viruses. Analysis of 38 samples from Sardinian cultivars for the presence GVG GVH was carried out using RT-PCR. All were also tested Pinot gris (GPGV) RT-PCR, leafroll -1, -2 -3, A (GVA) B (GVB), arabis mosaic (ArMV), fanleaf (GFLV) fleck (GFkV) multiplex confirmed in four vines, detected only one sample. In...

The ectoparasitic dagger nematode (Xiphinema index), vector of Grapevine fanleaf virus (GFLV), provokes gall formation and can cause severe damage to the root system of grapevines. Mycorrhiza formation by Glomus (syn. Rhizophagus) intraradices BEG141 reduced both gall formation on roots of the grapevine rootstock SO4 (Vitis berlandieri×V. riparia) and nematode number in the surrounding soil. Su...

The full-length transcript of grapevine fanleaf virus (GFLV) RNA2 produces a primary product of 122K when translated in the rabbit reticulocyte system. This 122K polyprotein is completely processed in vitro by the RNA1-encoded 24K proteinase. The positions of the cleavage sites within the polyprotein have been mapped and the genome organization of GFLV-F13 RNA2 has been established. The order o...

The putative 38 kDa movement protein (P38) gene, located on the RNA2 of grapevine fanleaf nepovirus (GFLV), was cloned in Escherichia coli and expressed as a fusion protein fused to six histidines (6HisP38). This protein was purified and used to produce a specific polyclonal antiserum from which immunoglobulins were isolated by immunoaffinity against the recombinant 6HisP38. Western immunoblot ...

Virus-like particles (VLPs) derived from nonenveloped viruses result from the self-assembly of capsid proteins (CPs). They generally show similar structural features to viral particles but are noninfectious and their inner cavity and outer surface can potentially be adapted to serve as nanocarriers of great biotechnological interest. While a VLP outer surface is generally amenable to chemical o...