Remodeling of glycans on the cell surface is an essential technique to analyze cellular function of lectin-glycan ligand interaction. Here we describe the methods to identify the responsible enzyme (glycosyltransferase) regulating the expression of the glycan of interest and to modulate the glycan expression by overexpressing the glycosyltransferase gene. For the identification of the responsib...

Glycosylation is a common modification reaction in plants. The products obtained upon glycosylation have different biological functions, making an important mechanism affecting and regulating the balance of plant growth metabolism. In this study, we first speculated that Group I apple glycosyltransferase family may predicted function like UGT83A1, according to gene chip data published online. S...

Plasmodesmata (Pd) are plant intercellular connections that represent cytoplasmic conduits for a wide spectrum of cellular transport cargoes, from ions to house-keeping proteins to transcription factors and RNA silencing signals; furthermore, Pd are also utilized by most plant viruses for their spread between host cells. Despite this central role of Pd in the plant life cycle, their structural ...