Eleven strains of lactobacilli were screened for their bile salt deconjugation ability, bile salt hydrolase activity (BSH) and coprecipitation of cholesterol with deconjugated bile. Bile salt deconjugation as determined by the release of cholic acid showed that more cholic acid was liberated from the deconjugation of sodium glycocholate than sodium taurocholate, and Lactobacillus acidophilus st...

Oleschuk, Curtis J., Roger G. Deeley, and Susan P. C. Cole. Substitution of Trp1242 of TM17 alters substrate specificity of human multidrug resistance protein 3. Am J Physiol Gastrointest Liver Physiol 284: G280–G289, 2003. First published October 9, 2002; 10.1152/ajpgi.00331.2002.—Multidrug resistance protein 3 (MRP3) is an ATP-dependent transporter of 17 -estradiol 17 (D-glucuronide) (E217 G)...

Taurocholate efflux was studied in Xenopus laevis oocytes and is consistent with a carrier-mediated process. This carrier can be competitively inhibited and trans stimulated by glycocholate. Transport is also trans stimulated by taurochenodeoxycholate and S-hexylglutathione, but not taurolithocholate or daunomycin, reflecting a range of specificity including substrates of both the hepatic canal...

I. The strength of binding of 64CU to the following was determined by dialysis against solutions of buffered sodium chloride with and without EDTA, penicillamine and sodium glycocholate. (a) Human albumin, serum and bile labelled in vitro from patients with and without Wilson's disease. (b) Rat bile labelled in vivo and in vitro. 2. Serum and albumin were found to bind copper comparatively weak...

Probiotic microorganisms are an indispensable part of a healthy life. The usage areas the probiotic Bacillus licheniformis bacteria quite wide, from contributing to development immune system reducing cholesterol, which is important cause cardiovascular diseases. In this study, (isolate 29) was isolated traditional yoghurt sample obtained town Hatipli/ Tokat/ Türkiye. And it’s genotypic and char...

Reflux of duodenal contents into the stomach has been implicated in the disruption of mucosal defence and the subsequent occurrence of gastric ulcer. The change produced in the rheological properties following the addition of bile salts and phospholipids to mucus samples was used to assess resultant structural changes. Sodium deoxycholate, sodium taurodeoxycholate, sodium glycocholate, and lyso...

F(+) strains of Escherichia coli infected with donor-specific bacteriophage such as M13 are sensitive to bile salts. We show here that this sensitivity has two components. The first derives from secretion of bacteriophage particles through the cell envelope, but the second can be attributed to expression of the F genes required for the formation of conjugative (F) pili. The latter component was...

PURPOSE Explore the usefulness of a perfusion system in order to establish human nasal epithelial cell cultures suitable for long-term in vitro ciliary beat frequency (CBF) and cilio-toxicity studies. METHODS The cells were obtained by protease digestion of nasal biopsy material. The cells were plated at a density of 0.8-1 x 10(6)/cm2 on Vitrogen-coated polyethylene terephthalate membranes, a...