Objective: To purify IgG molecules from several species by SpA-affinity chromatography and to study the interactions of mammalian IgGs with a peroxidase-labelled SpL, SpA and SpG conjugate (SPLAG-HRP) in an enzyme-linked immunosorbent assay (ELISA). Materials and methods: The periodate method described by Nakane and Kawoi was used to prepare the SPLAG-HRP conjugate. The 10% non-denaturing sodiu...

BACKGROUND We aim to demonstrate behavioral alterations in a sepsis model using intravenous (IV) immunoglobulin G (IgG) and immunoglobulins enriched with IgA and IgM (IgGAM). METHODS We divided 48 Wistar albino rats into five groups: control group, sham-operated group (only antibiotic treatment), cecal ligation and puncture (CLP) group (CLP plus antibiotic treatment), IgG group (250 mg/kg IV ...

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI ...

Immunoglobulins are major glycoproteins that modulate the immune response of gut-associated lymphoid tissue. In the present study, we sought to determine whether orally administered ovine serum immunoglobulins modulate selected indices of mucosal immune function and immune mediators in the growing rat challenged with Salmonella enteritidis. Rats were fed a casein-based basal control diet (BD; u...

background: drug use evaluation (due) aims at improving the patients’ care. studying the administration pattern of intravenous immunoglobulin (ivig) is an important research topic due to its significant role in the treatment and controlling of many disorders, high prices, and limited availability of this drug. methods: this observational cross-sectional study was conducted at shahid sadoughi ho...

The focus of this report was to assess the role of passive immunization using immunoglobulins derived from chicken egg yolk in protection against M. gallisepticum infection in chickens. Immunoglobulins were produced by egg yolk technology. ELISA was used to evaluate their immunoreactivity and western immunoblotting demonstrated that chicken egg yolk immunoglobulins Y (IgY) were capable of recog...

OBJECTIVE To estimate different proteins and immunoglobulins as humoral immune response in patients with intracranial tumours. METHODS Cellulose acetate membrane electrophoresis for estimation of different proteins and radialimmunodiffusion (RID) for measurement of serum and CSF immunoglobulins were used. RESULTS Thirty seven patients with different types of tumours showed increase in serum...

The mainstay of treatment of pemphigus vulgaris is systemic corticosteroids. Intravenous immunoglobulins have been reported as an adjuvant corticosteroid-sparing regimen in recalcitrant pemphigus vulgaris. The purpose of the study was to monitor disease activity, serum levels of autoantibodies and doses of oral corticosteroids in 4 patients with recalcitrant pemphigus vulgaris adjuvantly treate...