BACKGROUND Continuous amperometric sensors that measure glucose or lactate require a stable sensitivity, and glutaraldehyde crosslinking has been used widely to avoid enzyme loss. Nonetheless, little data is published on the effectiveness of enzyme immobilization with glutaraldehyde. METHODS A combination of electrochemical testing and spectrophotometric assays was used to study the relations...

Immunization with MFS-180 vaccine prepared in the presence of glutaraldehyde (0.05%), lipopolysaccharide (LPS) (100 micrograms/ml), and concanavalin A (Con A) (200 micrograms/ml) could protect Swiss mice against a subsequent challenge by 1 X 10(6) MFS-180 cells. The sequence of attachment of LPS and Con A to glutaraldehyde-treated cells as found to determine the efficacy of the vaccine. LPS cou...

The diffusion of glutaraldehyde from zinc oxide and eugenol cement (ZOE) into water was measured. The egress was found to be substantial and correlated to the amount of glutaraldehyde which was incorporated initially. The percentages of the fixative which diffused from the ZOE preparations containing 2,5, or 10% glutaraldehyde were virtually identical, demonstrating that there is no restriction...

Philasterides dicentrarchi is a protozoan ciliate which causes significant economic losses in fish aquaculture. This study investigated the effects of chitosan microspheres cross linked with glutaraldehyde and containing beta-cyclodextrin (βCD) on the survival of this parasite in 7 d cultures. When used alone in assays, neither chitosan nor βCD showed any activity, whereas free glutaraldehyde w...

This work assesses the effect of lyophilization on the cytotoxicity and residual aldehyde concentration of glutaraldehyde-treated and lyophilized bovine pericardium (group A), comparing it to conventional glutaraldehyde-treated bovine pericardium (group B). Cytotoxicity was measured by incubating a pericardium sample from each group in saline and assessing the eluant's influence on cellular gro...

  In the present study, the effect of stirring speed and the type of cross linking agent on the size and formation of microencapsulated Silver carp (Hypophthalmichthys molitrix) oil were investigated. The gelatin/gum Arabic was used for encapsulating and the capsules were prepared by complex coacervation. Microcapsules were analyzed by optical microscopy technique and particle size analyzer. Re...

The kinetics and isotherm studies of the immobilized lipase and the mechanism of immobilization on chitosan beads and activated chitosan beads with glutaraldehyde were investigated. The effect of glutaraldehyde on porosity of chitosan was evaluated by FESEM analysis. It was observed that the porosity of the carrier which has activated by glutaraldehyde was substantially increased. The validity ...

With increased use of glutaraldehyde fixatives (15), membrane whorls of myelin figures have been noted in many tissues prepared for electron microscopy (e.g ., 3, 7, 9, 10, 14, 16, 17) . Some workers have considered them artifacts produced by glutaraldehyde fixation (5, 12, 13) . We observed myelin figures in glutaraldehyde-osmium tetroxide-fixed conidia of Scopulariopsis brevicaulis, a member ...