Glycoside hydrolase family 13 contains exo-glucosidases specific for α-(1→4)- and α-(1→6)-linkages including α-glucosidase, oligo-1,6-glucosidase, and dextran glucosidase. The α-(1→6)-linkage selectivity of Streptococcus mutans dextran glucosidase was altered to α-(1→4)-linkage selectivity through site-directed mutations at Val195, Lys275, and Glu371. V195A showed 1300-fold higher kcat/Km for m...

A multicomponent protein complex containing manganese (II)-dependent peroxidase, laccase and beta-glucosidase was isolated from culture extracts of the white rot basidiomycete Lentinula edodes. This protein complex showed a single protein band on native polyacrylamide gel electrophoresis (PAGE). On sodium dodecyl sulfate (SDS)-PAGE, however, it displayed three major bands and several additional...

Glycogen autophagy in newborn rat hepatocytes was studied by using enzyme determinations and electron microscopy. Cyclic AMP induced glycogen autophagy in these cells. Glycogen-hydrolyzing acid glucosidase activity increased whereas acid mannose 6-phosphatase activity decreased in the liver of these animals. Parenteral glucose, which prevents postnatal glucagon secretion and tissue cyclic AMP e...

Previously, we isolated two α-amylase isozymes, HdAmy58 and HdAmy82, from the digestive fluid of the Pacific abalone Haliotis discus hannai (Kumagai et al., 2013, Comp. Biochem. Physiol., B 164, 80-88). These enzymes degraded starch producing maltooligosaccharides but not glucose. However, the digestive fluid itself could produce glucose from starch, indicating that the digestive fluid contains...

The effect of castanospermine (CSTP), an inhibitor of glucosidase I, on processing, activity, and secretion of lipoprotein lipase was studied in 3T3-L1 adipocytes. Processing was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of endoglycosidase H (endo H)-digested subunits of lipoprotein lipase from cells incubated 1-2 h with [35S]methionine. Lipoprotein lipase...

Using the longitudinal growth data of 13,809 individuals in Akita Prefecture, percentage distributions their adult height (AH) standard deviation scores (SDS) relation to prepubertal SDS were obtained. The AH increased with negative and decreased positive SDS, showing that a greater amount change was associated interval from mean. proportions who achieved normal stratified by as follows: 67.1%,...

β-Glucosidase is an important component of the cellulase complex. It not only hydrolyzes cellobiose and short-chain cellooligosaccharides to glucose, but also removes the inhibitory effect of cellobiose on the β-1, 4-endoglucanase and exoglucanase, thereby increasing the overall rate of cellulose biodegradation. β-glucosidasefrom culture supernatant of a fungus Penicillium simplicissimum was pu...

In this research, the influence of additives on rheological and sedimentary properties magnetorheological fluid (MRF) was tested analyzed. The were stearic acid, sodium dodecyl sulfate (SDS), their mixture, respectively. MRF composed carbonyl iron particle, silicone oil, liquid paraffin, graphite bentonite, SDS. results indicated that mainly influenced by mass fraction particle. When fractions ...

Abstract Proteins and amino acids are minor components of honey that rarely used for its quality evaluation, although these create biological activity as well can serve overheating detection. The aim the study was to use indicators confirm a declared on label variety. Fifty-eight samples 8 different varieties meeting commercial requirements were used, including 28 local Polish 30 (mainly import...

In this study, we investigated whether Sargassum yezoense extract (SYE) could inhibit α-glucosidase and α-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. Freeze-dried S. yezoense was extracted with 80% ethanol and concentrated for use in this study. The hypoglycemic effect was determined by evaluating the inhibitory activities of SYE a...