A new staining procedure for glucose oxidase at both the light and the electron microscopic level has been developed. The procedure was equally effective for the detection of galactose oxidase. It appears that the method can be applied to all oxidoreductases which catalyze the production of hydrogen peroxide. Aspergillus niger glucose oxidase and Dactylium dendroides galactose oxidase were used...

OBJECTIVE Hyperglycemia is the main determinant of long-term diabetic complications, mainly through induction of oxidative stress. NAD(P)H oxidase is a major source of glucose-induced oxidative stress. In this study, we tested the hypothesis that rosiglitazone (RSG) is able to quench oxidative stress initiated by high glucose through prevention of NAD(P)H oxidase activation. METHODS AND RESUL...

A number of nutritional factors influencing glucose oxidase (EC 1.1.3.4) production by Aspergillus niger NCIM 545 were studied. The synthesis of glucose oxidase by A. niger was investigated in two steps using submerged fermentation at 30±2 °C and 180 rpm for 96 h. Primarily, nutritional components were selected by onefactor-at-a-time method, and the significance of each component with respect t...

A novel GOD∼/AuNPs#rGO-CHIT/GC electrode was fabricated to realize the direct electron transfer of glucose oxidase (GOD) for rapid, highly sensitive and cost-effective determination BDE-15 based on blocking effect.

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H(2)O(2). The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid ...

In this study the effect of inoculum size, volume of fermentation medium and aeration rate was analyzed for UV mutated strain of Aspergillus niger AN-14 regarding enhanced production of glucose oxidase. UV treated 10 variants showing positive variations from parent strain were isolated and analyzed for glucose oxidase production using shake flask fermentation. Mutant strain AN-14 gave maximum p...

Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this stud...

Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on nonc...

The aim of the present study was to elucidate the intracellular mechanisms that cause neuronal cell death following exposure to excitatory neurotransmitter‑induced neurotoxicity, neurotoxins and oxidative stress. Human SH‑SY5Y neuroblastoma cells were exposed to various stimuli, including glutamate, 6‑hydroxydopamine (6‑OHDA), and glucose oxidase, and cell viability was determined by MTT assay....

the glucose oxidase (gox) electrochemical sensors were used to detect a little amount of glucose. in this paper, the pure platinum plate (length of 5 cm and a width of 3 mm), the ag/agcl electrode and platinum electrode (diameter of 1 mm) were applied as a working electrode (w.e), reference electrode and auxiliary electrode sequential. factors measured, glucose concentration and ph is phosphate...