Selected alkaloids are chromatographed on cyanopropyl-silica thin layers using various nonaqueous and aqueous eluents. Because of the strong retention of these basic compounds, nonaqueous eluents containing medium polar diluents, strongly polar modifiers, and silanol blockers (ammonia or diethylamine) are required for separation. Likewise, aqueous eluents containing modifiers (acetonitrile, met...

In continuation of Nepalese medicinal plant screening research, we have examined the antibacterial property of 40 methanol crude extracts in vitro using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. Overall, 95 % of the extracts showed antibacterial activity against at least one Gram-negative bacteria and 90 % of the extract...

The secondary metabolite constituents of Glaucium aleppicum Boiss. et Hausskn. ex Boiass. (Papaveraceae) were investigated using an in-house developed Ultra Performance Liquid Chromatography-Photodiode Array-High Resolution Tandem Mass Spectrometry (UPLC-PDAHRMS-MS/MS) method. In this study the powerful resolution and short analysis time afforded by UPLC coupled to the outstanding mass accuracy...

Glaucium flavum Crantz is a medicinal plant species which contains high variety of isoquinoline alkaloids, the main alkaloid glaucine, possessing antitussive activity. In vitro cultivation was investigated as an alternative and sustainable approach for this characterized by decreasing distribution. The biotechnological production secondary metabolites could be improved addition elicitors such j...

Alkaloids, plant secondary metabolites, have a wide variety of biological effects. For this reason, the extraction alkaloids from plants is strategic importance. Different protocols for been described by many authors. The objective study was to compare efficacy three different Glaucium corniculatum. This article compares Soxhlet and ultrasonication protocol, used in previous studies, modified p...