Giardia lamblia, a parasite of humans, is a major source of waterborne diarrhoeal disease. Giardia is also an excellent system to study basic biochemical processes because it is a single-celled eukaryote with a small genome and its entire life cycle can be replicated in vitro. Giardia trophozoites undergo fundamental changes to survive outside the intestine of their host by differentiating into...

This study was conducted to provide current information on the effectiveness of water treatment chemicals and filters for control of Giardia cysts in areas where treated water is not available. Four filters and seven chemical treatments were evaluated for both clear and turbid water at 10 degrees C. Three contact disinfection devices were also tested for cyst inactivation. Filters were tested w...

Giardia intestinalis is a protozoan parasite that causes giardiasis, a form of severe and infectious diarrhea. Despite the importance of the cell cycle in the control of proliferation and differentiation during a giardia infection, it has been difficult to study this process due to the absence of a synchronization procedure that would not induce cellular damage resulting in artifacts. We utiliz...

Background: Giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. Thus, in this study, the in vitro anti-giardial activity of chloroformic extract of Tanacetum parthenium and Artemisia annua on cyst and trophozoite of Giardia lamblia were separatel...

Giardia lamblia is present in the intestinal lumen as a binucleate, flagellated trophozoite or a quadranucleate, immotile cyst. Here we used the plant lectin wheat germ agglutinin (WGA), which binds to the disaccharide di-N-acetyl-chitobiose (GlcNAc(2)), which is the truncated Asn-linked glycan (N-glycan) of Giardia, to affinity purify the N-glycomes (glycoproteins with N-glycans) of trophozoit...

giardiasis caused by the intestinal flagellate giardia duodenalis. it is postulated that animals may be a reservoir for human infection and vice versa, thus, giardiasis classified as a zooanthroponotic disease. therefore, accurate information about the giardia infection in animals can help the control and prevention of disease in human. with respect to considerable population of cats in iran an...

UNLABELLED Encystation of the common intestinal parasite Giardia lamblia involves the production, trafficking, and secretion of cyst wall material (CWM). However, the molecular mechanism responsible for the regulation of these sequential processes remains elusive. Here, we examined the role of GlRac, Giardia's sole Rho family GTPase, in the regulation of endomembrane organization and cyst wall ...

The highly reduced protozoan parasite Giardia lamblia has minimal machinery for cellular processes such as protein trafficking. Giardia trophozoites maintain diverse and regulated secretory pathways but lack an identifiable Golgi complex. During differentiation to cysts, however, they produce specialized compartments termed encystation-specific vesicles (ESVs). ESVs are hypothesized to be uniqu...

Protein S-palmitoylation, a hydrophobic post-translational modification, is performed by protein acyltransferases that have a common DHHC Cys-rich domain (DHHC proteins), and provides a regulatory switch for protein membrane association. In this work, we analyzed the presence of DHHC proteins in the protozoa parasite Giardia lamblia and the function of the reversible S-palmitoylation of protein...

background: giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. thus, in this study, the in vitro anti-giardial activity of chloroformic extract of tanacetum parthenium and artemisia annua on cyst and trophozoite of giardia lamblia were separatel...