The locus ovarian tumor (otu) is involved in several aspects of oogenesis in Drosophila melanogaster. The possible role of otu in the determination of the sexual identity of germ cells has not been extensively explored. Some otu alleles produce a phenotype known as ovarian tumors: ovarioles are filled with numerous poorly differentiated germ cells. We show that these mutant germ cells have a mo...

The C. elegans germ line is generated by extensive proliferation of the two germ-line progenitor cells present in newly hatched larvae. We describe genetic and phenotypic characterization of glp-4, a locus whose product is required for normal proliferation of the germ line. glp-4(bn2ts) mutant worms raised at the restrictive temperature contain approximately 12 germ nuclei, in contrast to the 7...

this paper summarizes the proceedings of a 1 day national symposium entitled “reproductive biology, stem cells biotechnology and regenerative medicine” held at shahid sadoughi university of medical sciences, yazd, iran on 3rd march 2016. here, we collected the papers that presented and discussed at this meeting by specialists that currently researched about the overlaps of the fields of reprodu...

The germ cell nuclear factor (GCNF) is a member of the nuclear receptor super family of transcription factors. GCNF expression during gastrulation and neurulation is critical for normal embryogenesis in mice. GCNF represses expression of the POU domain transcription factor Oct4 during mouse post-implantation development in vivo. Oct4 is thus down-regulated during female gonadal development, whe...

background:gossypol is a yellow toxic pigment from the cottonseed that can cause acute or chronic toxicity in humans and animals by affecting the testicular tissues. nowadays cottonseed is used as food supplement for ruminants specially the sheep. in this study, two different stem cell lines of testicular tissue including gc1-spg (mouse testis) and sftf-pi43 (sheep testis) cells were used to ev...

Zygotically contributed ovo gene product is required for the survival of female germ cells in Drosophila melanogaster. Trans-allelic combinations of weak and dominant ovo mutations (ovoD) result in viable germ cells that appear to be partially transformed from female to male sexual identity. The O V O ” ~ mutation is partially suppressed by many Sex-lethal alleles that affect the soma, while th...

The germ line exhibits sexual dimorphism as do the somatic tissues. Cells with the 2X;2A chromosome constitution will follow the oogenic pathway and X;2A cells will develop into sperm. In both somatic and germ-line tissues, the sexual pathway chosen by the cells depends on the gene Sex-lethal (Sxl), whose function is continuously needed for female development. In the soma, the sex of the cells ...

Objective(s): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. Materials and Methods: ...

objective(s):mesenchymal stem cells (mscs) derived from wharton’s jelly (wj-mscs) are now much more appealing for cell-based infertility therapy. hence, wj-mscs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study. materials and methods: mscs were isolated from human wharton’s jelly and treated with bmp4, retinoic acid (ra) or co-cultured on human...