Castellaniella defragrans is a Betaproteobacterium capable of coupling the oxidation of monoterpenes with denitrification. Geraniol dehydrogenase (GeDH) activity was induced during growth with limonene in comparison to growth with acetate. The N-terminal sequence of the purified enzyme directed the cloning of the corresponding open reading frame (ORF), the first bacterial gene for a GeDH (geoA,...

Mini-Tn5-induced mutants with defects in utilization of linear terpenes such as citronellol, geraniol, citronellate and/or geranylate were isolated from Pseudomonas aeruginosa. One mutant was unable to utilize geraniol but showed wild-type growth with the three other acyclic terpenes tested. The Tn5 insertion site of the mutant was determined by DNA sequencing. Comparison with the P. aeruginosa...

Among insects, sexual pheromones are typically mixtures of two to several components, all of which are generally required to elicit a behavioral response. Here we show for the first time that a complete blend of sexual pheromone components is needed to elicit a response also in a butterfly. Males of the Green-veined White, Pieris napi, emit an aphrodisiac pheromone, citral, from wing glands. Th...

In this study, aerial parts of Lippia citriodora Kunth were harvested at three phenological stages (i.e. vegetative, full flowering and fruit set). The essential oil isolated by hydro distillation for 3 h using a Clevenger-type apparatus and the oil was analyzed by GC and GC-MS. The yields of oils (w/w%) in different stages was in the order of: full flowering (0.9%), vegetative (0.48%) and frui...

The chemical composition of the essential oils from aerial parts of Pectis apodocephala and Pectis oligocephala were analyzed by GC-MS. The essential oils of these species were predominantly constituted by monoterpenes. Geranial (42.9– 44.5%), neral (32.2– 34.2%) and α-pinene (10.7– 11.4%) were the main constituents in the oil of P. apodocephala, while p-cymene (50.3– 70.9%) and thymol (24.4– 4...

The chemical composition of the essential oils from aerial parts of Pectis apodocephala and Pectis oligocephala were analyzed by GC-MS. The essential oils of these species were predominantly constituted by monoterpenes. Geranial (42.9-44.5%), neral (32.2-34.2%) and alpha-pinene (10.7-11.4%) were the main constituents in the oil of P. apodocephala, while p-cymene (50.3-70.9%) and thymol (24.4-44...

Zingiber officinale Roscoe rhizome plays a vital role in food flavoring as well utilization folk medicine. Nepal is categorized among the leading countries of Z. production and export. Mature collection based on major sites within Nepal, but we are currently unaware chemical composition essential oil for A comparative investigation compositions, collected from 12 different eastern, mid, western...

The work presented in this thesis emphasizes the importance of considering oxidative activation in the toxicity assessment of fragrance chemicals. Compounds without contact allergenic properties can be activated either via autoxidation in contact with air or via cutaneous metabolism to reactive products which can cause contact allergy. It is important to prevent sensitization as the immunologic...