نتایج جستجو برای: gbs agar medium
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OBJECTIVE This study evaluated the accuracy of the commercial product Strep B OIA (optical immunoassay) compared to the standard agar and broth culture methods for detecting vaginal colonization with group B streptococcus (GBS). METHODS Preoperative vaginal cultures were obtained from 141 nonpregnant gynecological patients undergoing major gynecologic surgery. Major gynecologic surgery was de...
Development of rapid and sensitive detection methods for group B streptococci (GBS) in pregnant women remains useful in order to adequately identify pregnant women at risk of transferring GBS to their neonate. This study compared the CDC recommended sampling and culture method with two qPCR methods for detecting GBS colonization. For a total of 100 pregnant women at 35-37 weeks of gestation, on...
PROBLEM Group B Streptococcus (GBS) is a leading cause of neonatal morbidity and mortality. We tested the hypothesis that the choriodecidua plays a role in GBS-stimulated human beta defensin(HBD)-2 increases in amnion cells through a secreted factor of choriodecidual origin. METHOD OF STUDY Human amnion epithelial cells were treated with choriodecidual GBS-conditioned medium, live GBS, lipote...
Background and Aims: Group B streptococcus (GBS), is a bacterium that colonize in the vagina and/or rectum of pregnant, as well as non-pregnant women. The frequency of GBS varies in different geographical areas. Capsular serotyping of the bacterium could result in efficient vaccine designation. Serotyping data of GBS in Iranian pregnant women is limited. The aim of this study was to investigate...
The objectives were to determine the prevalence of group B streptococcus (GBS) and to characterize antibiotic resistance patterns. All pregnant women presenting to the triage units at two urban hospitals during three intervals from 2001 to 2004 were included. Each interval lasted approximately four weeks. Swabs were inoculated into selective broth and cultured on tryptic soy agar with 5% sheep ...
To examine the stability of repetitive-sequence (rep) PCR profiles, six species of bacteria were subcultured to blood agar plates and DNA was extracted from the cultures after 24, 48, and 72 h of incubation at 35 degrees C. In addition, the same species were subcultured to fresh blood plates daily and DNA was extracted from the cultures after growth of 5, 10, and 15 subcultures, respectively. r...
The following is an observation on the survival of dry cultures of pneumococci and streptococci for seven years under unusual and adverse conditions of storage. Dry cultures of 27 pneumococci and 10 streptococci prepared by the Division of Laboratories and Research of the Department of Health of New York (Wadsworth, 1939) were given to the author on 17th July, 1940. All the cultures were examin...
Introduction: Group B Strepococcus(GBS) is one of the most important causes of neonatal infections including septicemia and meningitis. It also plays an important role in maternal infections such as chorioamnionitis, pyelonephritis, metritis and post partum infection. The present study was carried out to find the prevalence of GBS in pregnant women with 28 to 38 weeks gestational age. Methods...
We compared StrepB Select medium (Select) after enrichment with conventional culture for the detection of Group B Streptococcus (GBS). Postenrichment sensitivities of Select and conventional culture were 98.8% and 92.2%, respectively (P<0.05). Select was superior for detection of GBS from vaginal-rectal specimens. Growth of non-GBS colonies required additional work to exclude the presence of GB...
INTRODUCTION During the last few decades, group B Streptococcus (GBS) has emerged as an important pathogen. The major reservoirs for GBS are the vagina and the peri-anal regions/rectum, and the colonization of these regions is a risk factor for subsequent infection in pregnant women and newborns. METHODOLOGY A prospective study was performed to determine the prevalence of GBS colonization in ...
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