Binding of cGMP to the GAF-B domain of phosphodiesterase 2A allosterically activates catalytic activity. We report here a series of mutagenesis studies on the GAF-B domain of PDE2A that support a novel mechanism for molecular recognition of cGMP. Alanine mutations of Phe-438, Asp-439, and Thr-488, amino acids that interact with the pyrimidine ring, decrease cGMP affinity slightly but increase c...

We report the X-ray crystal structure of a phosphodiesterase (PDE) that includes both catalytic and regulatory domains. PDE2A (215-900) crystallized as a dimer in which each subunit had an extended organization of regulatory GAF-A and GAF-B and catalytic domains connected by long alpha-helices. The subunits cross at the GAF-B/catalytic domain linker, and each side of the dimer contains in serie...

GAGA factor (GAF) binds to specific DNA sequences and participates in a complex spectrum of chromosomal activities. Products of the Trithorax-like locus (Trl), which encodes multiple GAF isoforms, are required for homeotic gene expression and are essential for Drosophila development. While homozygous null mutations in Trl are lethal, heterozygotes display enhanced position effect variegation (P...

To date eleven human PDE (3',5'-cyclic nucleotide phosphodiesterase) families have been identified. Of these, five families contain non-catalytic tandem GAF (cGMP-specific and -stimulated phosphodiesterases, Anabaena adenylate cyclases and Escherichia coli FhlA) domains, GAFa and GAFb, in the N-terminal part of the enzyme. For PDE2A, PDE5A and PDE6 the GAF domains have been shown to bind cGMP w...

OBJECTIVE To examine longitudinally the effects of Assertive Community Treatment (ACT) on Global Assessment of Functioning (GAF) scores in Edmonton, Alberta. METHODS We acquired GAF scores for all clients at initial registration in the ACT program and at subsequent 18- and 36-month time points while in ACT. We analyzed both the entire ACT cohort and separate diagnostic groups. RESULTS We ob...

Corrosion-resistance is the key to improve reliability and service lifespan of highly integrated reconfigurable filtering antennae. However, conventional methodology for corrosion prevention cannot achieve desired effects, due limited intrinsic corrosion-resistance capacity traditional metal-based devices. Here, we developed a antenna based on graphene assembled film (GAF), featuring significan...

This study used structural equation modeling (SEM) to evaluate the relative strength of associations between combat exposure, four posttraumatic stress disorder (PTSD) symptom factors, and functional impairment as indexed by Global Assessment of Functioning (GAF) scores in a sample of 315 veterans assessed at a Department of Veterans Affairs PTSD clinic. Results showed that the association betw...

Inspired by recent successes of deep learning in computer vision and speech recognition, we propose a novel framework to encode time series data as different types of images, namely, Gramian Angular Fields (GAF) and Markov Transition Fields (MTF). This enables the use of techniques from computer vision for classification. Using a polar coordinate system, GAF images are represented as a Gramian ...

Green autofluorescence (GAF) has been described in the short flagellum of golden and brown algae, the stigma of Euglenophyceae, and cytoplasm of different life stages of dinoflagellates and is considered by some researchers a valuable taxonomic feature for dinoflagellates. In addition, green fluorescence staining has been widely proposed or adopted to measure cell viability (or physiological st...

Background: The cyclic nucleotide phosphodiesterases PDE10 and PDE11 contain putatively regulatory GAF domains with unknown function. Results: Synthetic GAF domain ligands can activate both PDEs. Conclusion: PDE10 is activated by cAMP, whereas physiological ligand of the PDE11 GAF domains remains unknown. Significance: This is the first demonstration of a functional role of the PDE10 and PDE11 ...