This study was conducted to determine chromatin integrity and DNA damage by DNA electrophoresis and comet assays of buffalo fresh and frozen semen. Semen samples were collected from four buffalo bulls and evaluated after freezing for semen motility, viability, sperm abnormalities, chromatin integrity and DNA damage. A significant variation was found in semen parameters after thawing. Highly sig...

The current study was performed to test the of quality as well as the DNA integrity of preserved liquid and frozen buffalo spermatozoa due to addition of different concentrations of glutathione (0.50, 1.00, 2.00 and 3.00 mM). Liquid semen was preserved for 120 hour with or without addition of glutathione and examined for progressive motility, viability, plasma and acrosomal membranes integrity ...

A suitable method for the cryopreservation of donkey semen would be very valuable for the ex situ management of genetic diversity in this species. This report uses a variety of observation and trials to evaluate the effect of cryoprotectants in per-cycle pregnancy rates (PC) in equids females (jennies (donkey) and mares (horse)). This was explored by (1) comparing the results of insemination of...

Introduction Primary breeders are currently faced with the same problem plaguing most animal industries, rising production costs. Labor, feed and energy costs are forcing primary breeders to consider more economical ways of maintaining gene pools. One way to reduce such costs is with the use of frozen semen. With frozen semen available from superior sires, primary breeders will have the tool to...

Ram spermatozoa are most susceptible to damage during freezing between the temperatures of -10 degrees C and -25 degrees C. The objectives of the present study were to examine how freezing rate through this critical temperature zone affected the fertility of spermatozoa as assessed in vivo and in vitro. Semen from six adult rams was frozen at two different rates ("fast": 5 degrees C/min from +5...

Artificial insemination via cooled or frozen-shipped semen allows more mares to be bred to a single stallion, but not all stallions produce semen capable of withstanding the cooling and freezing procedures. Aurich et al. (1996) estimated that cooled or frozen-shipped semen achieves < 75% of the pregnancy rates achieved with fresh semen. Some factors affecting the fertility rates of thawed semen...

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen wa...

this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each...

BACKGROUND Artificial insemination (AI) can serve as a powerful tool to the sheep owners for making rapid genetic progress of their flock. The AI in sheep is mostly performed using fresh semen with two reasons i) lambing rate following trans-cervical AI with frozen semen is limited by the inability of frozen-thawed sperm to transit the cervix and ii) the need of circumventing the cervical barri...

Background: Semen cryopreservation is the most important section of artificial insemination programs; it allows preservation of semen fertility for a long time. Materials and Methods: The aim of the present study was to detect the effect of in vitro supplementation of 4-hydroxy Tempo on fresh and frozen spermatozoa quality of buffalo bulls. Five healthy buffalo bulls (5 ejaculates from each bul...