نتایج جستجو برای: freezing media

تعداد نتایج: 297753  

2018
Xiang Fan P. H. Diamond L. Chacón

Recent progress in the study of Cahn-Hilliard Navier-Stokes (CHNS) turbulence is summarized. This is an example of elastic turbulence, which can occur in elastic (i.e. self-restoring) media. Such media exhibit memory due freezing-in laws, as does MHD, which in turn constrains the dynamics. We report new results in the theory of CHNS turbulence in 2D, with special emphasis on the role of structu...

Journal: :TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series B 1991

Journal: :Journal of clinical microbiology 1976
E M Scott W Woodside

The effect of suspending media on the stability of pseudorabies virus upon freeze-drying and subsequent storage was studied. A variety of media was tested, including: sodium glutamate; sucrose; lactose; lactalbumin hydrolysate; peptone; a combination of sucrose, dextran, and glutamate; and various combinations of sucrose, glutamate, and potassium phosphates. Suspending media containing glutamat...

Journal: :International Journal of Solids and Structures 2015

2001
Ravi Radhakrishnan Keith E. Gubbins Malgorzata Sliwinska-Bartkowiak

Using molecular simulations and free energy calculations based on Landau theory, we show that freezing/melting behavior of fluids of small molecules in pores of simple geometry can be understood in terms of two main parameters: the pore width H* ~expressed as a multiple of the diameter of the fluid molecule! and a parameter a that measures the ratio of the fluid-wall to the fluid–fluid attracti...

Journal: :Cryobiology 2011
J Gadea M Molla E Selles M A Marco F A Garcia-Vazquez J C Gardon

In this study, total glutathione content was determined in human spermatozoa before and after cryopreservation. Total GSH in fresh semen was 4.47±0.46nmol/10(8) cells. Following semen cryopreservation, GSH decreased to 1.62±0.13nmol/10(8) cells, a 64% reduction (p<0.01). This decrease in GSH content was associated with a decrease in sperm progressive motility (68% of reduction, p<0.01). Additio...

A. Afshari A. Hatami G. Habibi K. Esmaeil Nia S. Bozorgi

The purpose of this study was to examine the potential application of Sorbitol, Sucrose and Gelatin in combination with glycerol in order to Theileria annulata infected cell line cryo-preservation. The efficiency of the freezing methods was compared by assessing the viability and plating efficiency of cells after resuscitation, dilution and equilibration in cell culture medium. As a result, the...

Today, sperm cryopreservation is a routine practice in infertility clinics. One of the alternative methods to preserve sperm is freeze-drying. This process consists of three steps. The first step is sample freezing, during which the solvent is separated from the solvent. Water forms ice crystals and the solute is placed between the ice crystals. Freezing may be done in a separate device or in a...

Journal: :Bioscience reports 2007
Boon Chin Heng Marie Veronique Clement Tong Cao

Previous study demonstrated that the low survival of human embryonic stem cells (hESC) under conventional slow-cooling cryopreservation protocols is predominantly due to apoptosis rather than cellular necrosis. Hence, this study investigated whether a synthetic broad-spectrum irreversible inhibitor of caspase enzymes, Z-VAD-FMK can be used to enhance the post-thaw survival rate of hESC. About 1...

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