A method is described for the separation of a diarrhoeal stool into solid and liquid phases. The seven patients studied had varying degrees of ileal resection. Faecal bile acids were measured in supernatant and pellet. Freeze-dried pellet of the patients had significantly higher bile acid content than freeze-dried faeces of the eight controls (patients mean = 55.8 mg bile acids/ g freeze-dried ...

The stabilities of eight strains of respiratory syncytial virus were compared after the strains were freeze-dried in the presence and absence of the stabilizer SPGA, which contains 218 mM sucrose, 7.1 mM dipotassium hydrogen phosphate, 3.76 mM potassium dihydrogen phosphate, 4.9 mM sodium glutamate, and 1% (wt/vol) bovine albumin. Strains freeze-dried in the presence of SPGA showed only small-t...

The avocado is a tropical fruit whose nutritional value is due mainly to its high lipid content. The aim of this work was to evaluate the rehydration of freeze-dried avocado (variety Collinson) powder and slices. The rehydration kinetics was determined using an experimental apparatus that monitored the volume of water absorbed by the sample with the time. Peleg and Weibull equations were used t...

The work reported here sought to assess the protection afforded by two stabilizing solutions (sorbitol-gelatin and glutamic acid-lactose) in preserving the potency of freeze-dried Schwarz strain measles virus during storage with a view to the production of reference preparations and working lots of virus suspensions. Stabilized virus suspensions and control suspensions were stored at -70 degree...

Foot-and-mouth disease virus, type A, strain 119, propagated in cultures of calf kidney cells and in the tongue epithelium of cattle was used. The process of freeze-drying was conducted in two cycles on unit volumes of 4 ml in Pyrex ampoules, averaging 150 ampoules per run, and was studied separately from the problems of storage. Ampoules containing freeze-dried virus were flame-sealed for eith...

OBJECTIVE The aim of this study was to fabricate a novel composite porous scaffold by blending chitosan and gamma-poly(glutamic acid) (gamma-PGA) for the sustained delivery of rhBMP-2. METHODS Chitosan and gamma-PGA were blended to fabricate a novel porous scaffold by the freeze-gelation method. For comparison, scaffolds made of freeze-dried chitosan, freeze-dried PLLA, and freeze-gelled chit...

In the present work the textural properties of mushroom and onion, fresh and after freeze-drying, were studied, to understand how this drying treatment influences the texture of these food products. The fresh products had average moisture contents of 90.25 % and 90.02 % (wet basis) and the freeze-dried ones had moisture contents of 7.01 % and 5.19 % (wet basis), for mushroom and onion, respecti...

PURPOSE To compare the effect of preserving sclera samples in either 95% ethanol or freeze-dried. METHODS Ninety-six samples of human sclera were studied. Half of them were freeze-dried and half preserved in 95% ethanol. Preservation periods of 18, 45, 90 or 174 days were studied. Automated immunostaining was carried out in the Ventana BenchMarkR LT platform using collagen 1 and fibronectin a...

OBJECTIVE To report clinical and radiographic graft incorporation capability of bovine freeze-dried bone grafts. METHODS Twenty five patients were enrolled. The mean follow-up was eight years. Grafts were purified and freeze-dried. Clinical analysis was based on the score of Merle d'Aubigné and Postel and an established score criteria for radiographic bone incorporation was used for radiograp...