AbstractThe ability of hesperidin (HP) to form complexes with five metals; cobalt, nickel, zinc, calcium and magnesium was investigated. The complexation was studied using U.V spectroscopic titration, in methanol as well as aqueous buffer solutions (physiological conditions). Potential complexes were studied by IR and NMR spectroscopy, melting point and their solubility were also evaluated. The...

A combination of a DNA ligase and a restriction endonuclease provides a DNA polymerase activity, which might suggest a novel strategy for polymer synthesis.

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...

The palladium nanoparticles were immobilized on DNA-modified multi walled carbon nanotubes as stable and powerful heterogeneous catalyst. The catalyst was characterized by FT-IR spectroscopy, UV-Vis spectroscopy, field emission scanning electron microscopy, X-ray diffraction, transmission electron microscopy, inductively coupled plasma and elemental analysis. DNA as a well-defined structure and...

n page 993, Bucciarelli et al. report the first evidence that chromosomes are not always necessary for proper spindle assembly and cell division. The authors stumbled upon chromosome-independent cell division during a screen for meiosis mutants in Drosophila. Two of the mutants they identified, fusolo and solofuso , had chromosome segregation defects that led to secondary spermato-cytes lacking...

Supercoiling is introduced into DNAmolecules when the double helix is twisted around its own axis in three-dimensional space. Generally, DNAmolecules are negatively supercoiled inside cells, although the level of supercoiling is not equal throughout the genome and many supercoils may be constrained by bound proteins. Supercoiling increases the free energy of DNA and influences DNA metabolism by...

Abstract Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) as input signal fluorescence output signal, if DNA (DNA-L), (DNA-S), ATP were present at the same time, on...

abstractthe ability of hesperidin (hp) to form complexes with five metals; cobalt, nickel, zinc, calcium and magnesium was investigated. the complexation was studied using u.v spectroscopic titration, in methanol as well as aqueous buffer solutions (physiological conditions). potential complexes were studied by ir and nmr spectroscopy, melting point and their solubility were also evaluated. the...