Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. mul...

Turkeys vaccinated with various experimental vaccines and a commerical bacterin for fowl cholera and surviving an artificially induced epornitic were killed, and their carcasses were examined for wholesomeness. It was evident that, if the "fitness for human consumption" judgement was considered in addition to mortality, efficacy ratings of the various vaccines changed. This suggests that the "f...

capsules from a range of pathogenic bacteria are the key determinants of virulency. the capsule hasbeen implicated in virulence of pasteurella multocida. in this study a type-specific polymerase chain reaction(pcr) assay was used for capsular typing of 39 avian p. multocida isolates from iran. the pcr amplified afragment of 1044 bp from all of tested isolates. it was found that all avian p. mul...

1 WOULD LIKE TO REITERATE THE following results, which I have previously had the honor of presenting to the academy: 1. Fowl cholera is a virulent disease of the first order. 2. The virus * consists of a microscopic parasite which multiplies readily in culture away from the animal body: From this it IS possible to obtain the virus in a state of purity and demonstrate irrefutably that it is the ...

Outer Membrane Protein (OMP) enriched extracts of Pasteurella multocida A:1 (“A”) and local isolate (“L”) obtained from Nutrient rich free cell (FC), Nutrient deficient free cell (DC) and Biofilm cell (BC) growth were analyzed by SDS-PAGE. In general, the OMP profile in all the three growth conditions of “A” was different from that of “L” suggesting that both could be of different serotype. The...

A 5-yr retrospective study (November 2006-December 2011) was conducted to determine the isolation frequency of Pasteurella multocida and Gallibacterium anatis and their antibiograms from chickens submitted to the Mississippi Poultry Research and Diagnostic Laboratory. The number of isolations of G. anatis increased over the last 5 yr in broiler and broiler breeder type chickens. For P. multocid...

The effectiveness of an injected caprine serum fraction-immunomodulator (CSF-I2) as an immunostimulant in male and female F-line and commercial turkey poults infected with fowl cholera (Pasteurella multocida) was examined in separate trials. In the first 2 of 3 controlled trials, the effects of an i.m. injection of CSF-I2 given 24 h prior to a P. multocida challenge, administered by s.c. inject...

A Pasteurella multocida species-specific oligonucleotide probe, pmhyb449, targeting 16S rRNA was designed and evaluated by whole-cell hybridization against 22 selected reference strains in animal tissues. It differentiated P. multocida from other bacterial species of the families Pasteurellaceae and Enterobacteriaceae and also from divergent species of the order Cytophagales (except biovar 2 st...

We measured the humoral immune response in chickens against a formalin-inactivated alum-precipitated fowl cholera vaccine. The vaccine was administered in 12 weeks old chickens, 5×10 7 CFU/ml/chicken intramuscularly. Booster dose was given with similar dose and route at 15, 30 and 45 days intervals in groups A, B and C, respectively, after primary vaccination. The group D served as unvaccinated...