The nitro blue tetrazolium test was applied on neutrophils from children with kwashiorkor and from control children. The percentage of formazan cells formed in the kwashiorkor patients was significantly less than in the control group in spite of the presence of associated infections in the former. A direct correlation exists between the percentage of formazan cell formation and total serum prot...

In the title compound, C(15)H(15)N(5)O(2), the nitro O atoms are disordered over two sets of sites with an occupancy ratio of 0.75 (4):0.25 (4). Amine-imine tautomerism is observed in the formazan group. This was evident from the similar C-N bond distances in the formazan [1.319 (2) and 1.332 (3) Å], as well as the distribution of the imine proton in the Fourier difference map which refined to ...

The tetrazolium dye, 3-(4,5-dimethylthia/ol-2-yl)-2,5-diphi-iiyltclrazolium bromide (MTT), is reduced by live but not dead cells, and this reaction is used as the end point in a rapid drug-screening assay. It can also be used for accurate determinations of drug sensitivity but only if a quantitative relationship is established between cell number and M l'I forma/an production. We have shown tha...

This study assessed the growth characteristics and proteoglycan (PG) production in vitro of cells isolated from various zones; annulus fibrosus (AF), the transitional zone (TZ, an opalescent region situated between the AF and nucleus pulposus, NP) and the NP of the ovine intervertebral disc. The isolated cells were subsequently cultured in calcium alginate microspheres. Cellular metabolic activ...

A new tetrazolium salt XTT, sodium 3'-[1-[(phenylamino)-carbonyl]-3,4-tetrazolium]-bis(4-methoxy-6- nitro)benzene-sulfonic acid hydrate, was evaluated for use in a colorimetric assay for cell viability and proliferation by normal activated T cells and several cytokine dependent cell lines. Cleavage of XTT by dehydrogenase enzymes of metabolically active cells yields a highly colored formazan pr...

SASAI, Y. Lactic Dehydrogenase Activity in Human Epidermis: Semiquantita tive Histochemical Study. Tohoku J. exp. Med ., 1972, 108 (4), 327-336 The graphical method of Lineweaver-Burk to the histoehemical system was applied to obtain quantitative information on the lactic dehydrogenase activity in human epidermis. In vitro experiment showed that the incubation time necessary for yielding a cert...

By ALBINA ANN YAKAITIS G RANULES of formazan resulting from tile reduction of neotetrazolium have been observed in leukocytes by Wachstein) Studies were made on rats, mice, guinea pigs, rabbits and on normal and leukemic humans. In normal human material, formazan formation was noted principally in the mature myeloid elements and in varying degrees in lymphocytes and monocytes. In myelocytic leu...