Phospholipase D plays a key role in the biosynthesis of phosphatidic acid, a second messenger involved in essential cellular processes. Oleate-activated phospholipase D was the first mammalian phospholipase D isoform to be discovered but is the least known. The study was aimed to test a fluorometric method of assessment of oleate-activated phospholipase D activity in different biological materi...

We evaluated the usefulness of a fluorometric method for determining serum retinol (Futterman et al., Invest Ophthalmol Vis Sci 1975;14:125-30) in which fluorescence (excitation 335 nm; emission 460 nm) of retinol is directly measured in unextracted, diluted serum. Using serum from 466 individual donors, we compared values so obtained with those by a "high-performance" liquid-chromatographic me...

A rapid and simple assay method for determining neuraminidase activity was investigated fluorometrically with 4-methylumbelliferyl-N-Ac-a-D-neuraminide as a substrate. The time required for performance was less than 3 hr and the activity could be simply measured with a fluorescent spectrophotometer. Neuraminidase activity in culture supernatant of MDCK cell infected with a small quantity of inf...

A semiautomated, enzymatic, fluorometric method for the determination of the low glucose concentrations normally present in urine from subjects in the fasting state is described. The method is developed from a manual, enzymatic, fluorometric technic for the determination of urinary glucose using hexokinase (HK) and glucose-6phosphate dehydrogenase (G-6-PDH). The procedure is based on measuremen...