Optical anisotropy in a new form of crosslinked liquid crystal network incorporating a fluorescent chromophore has been demonstrated. Linearly polarised absorption and fluorescence is observed in oriented thin films. From measurement of absorption and fluorescence anisotropy, the order parameter was found to be 0.65 and 0.80, respectively. Temperature dependent birefringence measurements reveal...

Chapter Outline Introduction 105 Multi-Parameter Microscopy of Protein Aggregation Kinetics 106 Fusion Protein Labeling of Amyloidogenic Proteins 106 Principle of Multi-Parameter Imaging Techniques 107 Fluorescence Anisotropy Imaging 107 Principle of HomoFRET in the Study of Protein SelfAssembly Reactions 107 HomoFRET Imaging for the Study of α-synuclein Aggregation 108 Fluorescence Lifetime Im...

Human sera from Pneumocystis carinii-infected patients and specific rabbit antisera have antibodies against the cyst form of the organism. Lung tissue concentrations from cortisone-treated C3H/HeN mice and six open lung biopsy-positive patients were centrifuged and suspended, and immunofluorescent staining was done. We utilized the fluorescence-activated cell sorter to analyze and sort P. carin...

the changes of chl a fluorescence induction curve in response to different salinity levels in canola (barassica napus, l.) plants were investigated. the canola seedlings were exposed to salt stress (100 mm and 200 mm nacl) for 7 days and chl a fluorescence induction kinetics was analysed. by using the ojip-test, various parameters like vj, area, n, φpo, φeo, ψo, rc/abs and piabs were calculated...