The active, stretched conformation of the RecA filament bound to single-stranded DNA is required for homologous recombination. During this process, the RecA filament mediates the homology search and base pair exchange with a complementary sequence. Subsequently, the RecA filament dissociates from DNA upon reaction completion. ATP binding and hydrolysis is critical throughout these processes. Li...

We investigate, using molecular dynamics, how the severing protein, actin depolymerization factor (ADF)/cofilin, modulates the structure, conformational dynamics, and mechanical properties of actin filaments. The actin and cofilactin filament bending stiffness and corresponding persistence lengths obtained from all-atom simulations are comparable to values obtained from analysis of thermal fluc...

Actin filament formation and turnover within the treadmilling actin filament array at the leading edge of migrating cells are interdependent and coupled, but the mechanisms coordinating these two activities are not understood. We report that Coronin 1B interacts simultaneously with Arp2/3 complex and Slingshot (SSH1L) phosphatase, two regulators of actin filament formation and turnover, respect...

Actin filament dynamics govern many key physiological processes from cell motility to tissue morphogenesis. A central feature of actin dynamics is the capacity of filaments to polymerize and depolymerize at their ends in response to cellular conditions. It is currently thought that filament kinetics can be described by a single rate constant for each end. In this study, using direct visualizati...

We report on deep XMM-Newton observations of the vertex filament in the southern giant lobe of the Fanaroff-Riley class I radio galaxy Centaurus A. We find no X-ray excess from the filament region and place a 3σ upper limit on the 1 keV flux density of the filament of 9.6 nJy. This directly constrains the electron density and magnetic field strength in the filament. For the first time in an ind...

Fused filament fabrication (FFF) is one of the most popular additive manufacturing (AM) processes that utilize thermoplastic polymers to produce three-dimensional (3D) geometry products. The FFF materials have a significant role in determining properties final part produced, such as mechanical properties, thermal conductivity, and electrical conductivity. This article intensively reviews state-...

© Appl. Rheol. 19 (2009) 41969 Abstract: Filament breakup of high viscosity fluids with apparent yield stress has been investigated and strategies for an appropriate characterization of their behavior in CaBER experiments are discussed. Filament profiles of such fluids exhibit significant concave curvature. Accurate determination of filament shape is mandatory for understanding deformation beha...

In this paper we investigate the stability of a straight vortex filament with phase twist described by the three-dimensional complex Ginzburg-Landau equation ~CGLE!. The results of the linear stability analysis show that the straight filament is stable in a limited region of the two parameter space of the CGLE. The stable region is dependent on the phase twist imposed on the filament and shrink...