Fibroblast activation and proliferation are important for fibroblast-myofibroblast transdifferentiation, a crucial process in the pathological changes that define renal interstitial fibrosis. The left-right determination factor (Lefty) is an important cytokine of the transforming growth factor (TGF)-β family, with two variants, Lefty-1 and Lefty-2, in mice. Lefty has diverse functions, such as ...

Stem cell fate is largely determined by cellular signaling networks and heavily dependent on the supplementation of exogenous recombinant proteins into culture media; however, uneven distribution inconsistent stability are closely associated with spontaneous differentiation pluripotent stem cells (PSCs) result in significant costs large-scale manufacturing. Here, we report a novel PSC system vi...

Chronic kidney disease is a major cause of death, and renal fibrosis is a common pathway leading to the progression of this disease. Although activated fibroblasts are responsible for the production of the extracellular matrix and the development of renal fibrosis, the molecular mechanisms underlying fibroblast activation are not fully defined. Here we examined the functional role of AMP-activa...

ethanol known as ethyl alcohol is being widely used around the world. many serious diseases are related with its consumption.  alcohol posses many divers effects on human body including risk of cirrhosis and/or hepatocellular carcinoma. therefore, analysis of this component is prominent. fibroblast cells were cultured in various dosages of ethanol. the effective dosage was then investigated by ...

Chronic kidney disease is a global epidemic that affects estimated 10% of the world population. As the final common pathway of chronic kidney diseases, renal fibrosis can result in massive destruction of normal kidney structure and subsequent impairment of the function. Activated fibroblasts are recognized to be principal mediators of renal fibrosis. Unlike in physiological wound repair, where ...

Abstract SERS tags are a class of nanoparticles with great potential in advanced imaging experiments. The preparation however is complex, as they suffer from the high variability signals observed even at slightest sign aggregation. Here, we developed method for based on use gold nanostars conjugated neutravidin. here obtained extremely stable all biological buffers commonly employed and can be ...