in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

Tissue-specific stem cells found in adult tissues can participate in the repair process following injury. However, adult tissues, such as articular cartilage and intervertebral disc, have low regeneration capacity, whereas fetal tissues, such as articular cartilage, show high regeneration ability. The presence of fetal stem cells in fetal cartilaginous tissues and their involvement in the regen...

human mesenchymal stem cells (hmscs), which also called mesenchymal stromal cells, are multipotent stem cell. human mscs typically are positive for the surface markers cd44, cd73, cd90, cd105, cd106, and also negative for hematopoietic markers cd34 and cd45.these cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...

neurotrauma is one of the main medical problems that affect considerable number of population especially young persons in all countries. neurotrauma not only is a serious threat for life but also creates various disabilities and consequently imposes large costs to society. following central nervous system (cns) injury, tissue damage occurs via primary and secondary mechanisms. despite many atte...

BACKGROUND Hematopoietic progenitors are generated in the yolk sac and aorta-gonad-mesonephros region during early mouse development. At embryonic day 10.5 the first hematopoietic stem cells emerge in the aorta-gonad-mesonephros. Subsequently, hematopoietic stem cells and progenitors are found in the fetal liver. The fetal liver is a potent hematopoietic site, playing an important role in the e...

background: umbilical cord tissue is a very rich source of mesenchymal stem cells. instead of discarding this source we are banking the tissue along with cord blood for possible future cell based applications. the cord tissue needs to be transported and stored properly in order for it to be good enough for cell isolation at a later date. in this paper we have carried out a validation study to d...

introduction : use of adipose-derived stem cells (adscs) is a suggested hypothesis to enhance the regeneration of bone defects. this technique is of particular interest since adscs are more easily accessible via a non-invasive harvesting technique compared to bone marrow stem cells (bmscs). this study sought to assess the efficacy of adsc implantation for regeneration of localized mandibular bo...

BACKGROUND We have investigated the usefulness of a model of cardiac development in a large mammal, sheep, for studies of engraftment of human stem cells in the heart. METHODS AND RESULTS Adult and fetal human mesenchymal stem cells were injected intraperitoneally into sheep fetuses in utero. Hearts at late fetal development were analyzed for engraftment of human cells. The majority of the en...

Cardiovascular disease remains the leading cause of morbidity and mortality in the United States and Europe. In recent years, the understanding that regenerative processes exist at the level of the myocardium, has placed stem cell research at center stage in cardiology. A stem cell is a cell that has the ability to divide (self replicate) for indefinite periods often throughout the life of the ...