Regulation and maximization of lipase production in a mutant derivative of R. oligosporus has been investigated using different substrates, inoculum sizes, pH of the medium, temperature, and nitrogen sources in shake flask experiments and batch fermentation in a fermentor. The production of intracellular lipase was improved 3 times following medium optimization involving one-at-a-time approach ...

The yeast Pachysolen tannophilus was entrapped in calcium alginate beads to ferment D-xylose on a continous basis in the presence of high cell densities. Experimental operating variables included the feed D-xylose concentration, the dilution rate, and the fermentor biomass concentration. Under favorable operating conditions, cultures retained at least 50% of their initial productivity after 26 ...

The work describes the production of alkaline protease in a lab scale fermentor from Bacillus subtilis IH-72. The production of alkaline protease was enhanced by optimization of cultural conditions in a stirred fermentor. The alkaline protease so produced was applied to the goat skin for the removal of hair. The skin pieces were treated in three different ways; singly with the enzyme, in combin...

Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant strains screened, one mutant, Streptomyces ambofaciens XC 2-37, produced 9% more spiramycin than the parent strain S. ambofaciens XC 1-29. The effects of soybean oil and propyl alcohol on spiramycin production with S. ambofaciens XC 2-37 were studied. The potency of S. ...

the present investigation was aimed at studying the effect of incubation period, media, vitamins and solvents on biotransformation of albendazole by cunninghamella blakesleeana. the transformation was evaluated and identified by high performance liquid chromatography (hplc) and the structures of the transformed products were assigned by liquid chromatography-tandem mass spectrometry (lc/ms/ms) ...

Objective: B.subtilis and B.licheniformis have been reported as one of the potential protease producer. Hence, an attempt has been made to optimize and compare the enzyme production by entrapment and fermentor conditions. Methods: Growth profile of both the strains has been studied. Optimization of culture conditions with respect to pH, temperature, agitation, carbon, nitrogen sources and metal...