Fed-batch cultures are used in producing monoclonal antibodies industrially. Existing protocols are developed empirically. Model-based tools aiming to improve productivity are useful with model reliability and computational demand being important. Herein, a systematic framework for developing predictive models is presented comprising of model development, global sensitivity analysis, optimal ex...

Industrial therapeutic protein production has been greatly improved through fed-batch development. In this study, improvement to the productivity of a tissue-plasminogen activator (t-PA) expressing Chinese hamster ovary (CHO) cell line was investigated in shake flask culture through the optimization of the fed-batch feed and the reduction of ammonia generation by glutamine replacement. The t-PA...

Abstract: In this paper an optimal control algorithm for E. coli fed-batch fermentation has been developed. A simple material balance model is used to describe the E. coli fermentation process. The optimal feed rate control of a primary metabolite process is studied and a biomass production is used as an example. An optimization of a fed-batch fermentation process is usually done using the calc...

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase p...

A comparative study of software sensors using Multiway Partial Least Squares and Extended Kalman Filters in an application to a fed-batch yeast fermentation process is presented. The MPLS theory is introduced firstly and then applied to a yeast fed-batch fermentation process to provide soft-sensing facilities. The soft-sensing capabilities of the MPLS approach are found to compare favourably wi...

A novel optimal control algorithm is presented for application to batch processes. The algorithm utilises a linear model of the process that is identified using multi-way partial least squares. This linear model is used within an optimal control law to regulate the productivity of the batch by manipulating the substrates that are fed into the fermentation vessel. The proposed control algorithm ...

BACKGROUND The initial part of process development involves extensive screening programs to identify optimal biological systems and cultivation conditions. For a successful scale-up, the operation mode on screening and production scale must be as close as possible. To enable screening under fed-batch conditions, the membrane-based fed-batch shake flask was developed. It is a shake flask mounted...

To intensify the brewing process and increase the fermenter productivity, many breweries throughout the world have been embracing high-gravity brewing. However, increasing original wort concentration can cause a negative effect on yeast fermentation performance. This study focused on the application of fed-batch fermentation in high-gravity brewing. High gravity worts of 24oBx (using 30% high m...