Impaired secretion of lithostathine, a pancreatic glycoprotein capable of inhibiting the growth of CaCO3 crystals, has been reported in chronic calcifying pancreatitis. Controversial results were obtained, however, using immunoassays with different antibodies. The aim of this study was to purify and to measure juice lithostathine by a non-immunological method. Fast protein liquid chromatography...

Brugia malayi microfilarial excretory-secretory (mf ES) and phosphate buffer saline soluble (mf S) antigens were fractionated by fast protein liquid chromatography (FPLC) on superdex 200 HR 10/30 gel filtration column. The active antigen fractions were identified and explored in comparison with whole mf ES and mf S antigens to detect filarial IgG antibodies in different groups viz microfilaraem...

background and objectives: furfural (f) and hydroxymethyl furfural (hmf) are cyclic aldehydes, which are formed during the heat processing of foods. these chemical contaminants have received much attention due to their suspected health hazards and heat damage indicators. the aim of the present study was extraction and determination of f and hmf in baby-foods using dispersive liquid-liquid micro...

An endopeptidase was purified to homogeneity from the cell extracts of Treponema denticola ATCC 35405 (a human oral spirochete) by a procedure that comprised dialysis, anion exchange fast protein liquid chromatography (FPLC), hydroxylapatite FPLC, immobilized metal affinity FPLC, FPLC chromatofocusing, and two consecutive gel permeation FPLC steps. The enzyme is a 62-kDa protein with an isoelec...

The two 4.6 kb chloramphenicol resistance (CmR) plasmids pSCS6 and pSCS7, previously identified in Staphylococcus aureus from subclinical bovine mastitis, both encoded an inducible chloramphenicol acetyltransferase (CAT, EC 2.3.1.28). The pSCS6- and pSCS7-encoded CAT variants were purified by ammonium sulphate precipitation, ion-exchange chromatography and fast protein liquid chromatography (FP...

Two novel extracellular keratinases were produced by Actinomadura keratinilytica strain Cpt20. Both enzymes purified to homogeneity using heat-treatment (60 °C for 30 min) and ammonium sulfate salt fractionation (40 %–70 %), followed anion-exchange chromatography with fast protein liquid (FPLC) system. The keratinases, designated as KERA-71 KERB-19, are monomeric named according their molecular...

conclusions this method could be feasibly used for purification of large quantities of allicin from garlic extract. results semi-preparative hplc yielded a distinct peak with a retention time of 12.28 minutes. the purity (≥ 95%) and biological activity (p > 0.05) of purified allicin were comparable to the standard allicin. purified allicin produced same peak areas compared to the standard allic...

Neuropeptide Y (NPY) is a 36 amino acid peptide found throughout the central and peripheral nervoussystem of rat and human. NPY has been proposed to play an important role in satiety. The aim of this studywas to produce cell lines that secrete high levels of bioactive NPY. For this purpose, the complementaryDNA (cDNA) that encodes NPY was isolated by PCR. The cDNA was then clo...

in the present study, a comprehensive and systematic strategy was described to evaluate the performance of several three-way calibration methods on a bio-analytical problem. parallel factor analysis (parafac), alternating trilinear decomposition (atld), self-weighted alternating trilinear decomposition (swatld), alternating penalty trilinear decomposition (aptld) and unfolded partial least squa...