Excessive circulating triglycerides due to reduction or loss of lipoprotein lipase activity contribute to hypertriglyceridemia and increased risk for pancreatitis. The only gene therapy treatment for lipoprotein lipase deficiency decreases pancreatitis but minimally reduces hypertriglyceridemia. Synthesized in multiple tissues including striated muscle and adipose tissue, lipoprotein lipase is ...

Primary Hyperlipoproteinaemias Hyperchylomicronaemia (familial fatinduced hypertriglyceridaemia). This condition, which is characterized by gross accumulation of chylomicrons in the serum due to defective clearing of dietary fat, was first described by Burger and Grutz in 1932. Cases have since been recorded under a variety of names, the most common being 'idiopathic familial hyperlipaemia' and...

Lipoprotein lipase synthesis in adipose tissue was greater in rats fed ad libitum or refed than in fasted rats. Insulin alone and together with dexamethasone increased lipoprotein lipase synthesis in adipose tissue incubated in vitro. The changes in relative lipoprotein lipase synthesis (immunoprecipitable 35S-labelled lipoprotein lipase as a fraction of general [35S]protein after pulse-labelli...

Addition of heparin to isolated fat cells incubated at 23” resulted in a 3-fold increase in release of lipoprotein lipase activity into the incubation medium. This increase in medium lipoprotein lipase activity was not due to enhancement or stabilization of lipase activity present in the medium. The maximum increase in lipoprotein lipase activity was obtained on addition of 0.1 unit of heparin ...

The Blueprint Genetics Hyperlipidemia Panel is ideal tool for genetic analysis of patients with virtually any type of monogenic (Mendelian inheritance) dyslipidemia disorder. All classical familial hypercholesterolemia (FH) genes (APOB, LDLR, LDLRAP1 and PCSK9) as well as genes associated with other hyperlipidemias such as ‘type 3 hyperlipidemia’ (APOE), sitosterolemia (ABCG5, ABCG8), lipoprote...

Lipoprotein lipase activity in adipose tissue responds rapidly to changes in the physiological state. To study what mechanisms are involved in the regulation, guinea pigs were fasted and the decrease in adipose-tissue lipoprotein lipase activity was compared with the decreases in mRNA and lipase synthesis. The mRNA pattern (three species) did not change. There was a close parallelism between th...

Addition of heparin to isolated fat cells incubated at 23” resulted in a 3-fold increase in release of lipoprotein lipase activity into the incubation medium. This increase in medium lipoprotein lipase activity was not due to enhancement or stabilization of lipase activity present in the medium. The maximum increase in lipoprotein lipase activity was obtained on addition of 0.1 unit of heparin ...

OBJECTIVE We investigated the impact of lipoprotein lipase (LPL) gene mutations on apolipoprotein B (apoB)-100 metabolism. METHODS AND RESULTS We studied 3 subjects with familial LPL deficiency; 14 subjects heterozygous for the LPL gene mutations Gly188Glu, Trp64Stop, and Ile194Thr; and 10 control subjects. Very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), and low-d...

It has been postulated that lipoprotein lipase, an enzyme important in the uptake of fatty acids into tissues, is bound to the vascular endothelial cell surface and that this binding occurs through attachment to heparinlike glycosaminoglycans. Furthermore, it is thought that heparin releases the enzyme from its attachment to the endothelium into the circulation. These hypotheses have never been...