BACKGROUND Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting analysis and controlled reaction conditions. METHODS We designed 4 amplicons b...

normal hemostasis requires balanced regulation of prothrombotic and antithrombotic factors. inherited alteration of factor v and prothrombin gene, the g20210a mutation, increases the resistance of factor v to degradation and booster production of prothrombin respectively. these alterations can increase hypercoagulability leading to thrombotic consequences. we aimed to assess the frequencies of ...

conclusions the prevalence of both fv and fii variants are population based. iran is an ethnically diverse country. therefore, for a comprehensive analysis of a potential association of fv and/or fii mutations with stroke among iranian population, epidemiological studies could be conducted among different ethnic groups. patients and methods the study population consisted of 153 patients of diff...

background: role of genetic factors in etiology of preeclampsia is not confirmed yet. objective: gene defect frequency varies in different geographic areas as well as ethnic groups. in this study, the role of factor v leiden mutation in the pathogenesis of preeclampsia syndrome among the pregnant population of northern shore of persian gulf in iran, were considered. materials and methods: betwe...

factor v leiden deficiency is the most common hereditary hypercoagulable disease in the united states and involves 5% of the caucasian population. up to 30% of patients who present with deep vein thrombosis (dvt) or pulmonary thromboembolism present with this condition. this is a case report of a 36-year-old man who experienced one episode of dvt within the previous year and was admitted to our...