A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

A simple and rapid analytical method was established for detection of anabolic agents, zeranol and alpha-trenbolone and beta-trenbolone residues in cattle muscle and liver. This method consisted of aqueous acetonitrile extraction and successive solid-phase extraction with a tandem connected octadecyl (C18)-aminopropyl (NH2) cartridge, followed by on-line clean-up using multidimensional HPLC wit...

Extraction and purification of Anthocyanin pigments from Black Raspberries (Rubus occidentalis) was successfully carried out. Homogeneous and fine powder was obtained from the liquid nitrogen blending process. After extraction and purification of the plant material, a colored solution was obtained. Depending on the nature of the predominant anthocyanin and its concentration, the coloration of t...

A reversed-phase high-performance liquid chromatography method using evaporative light-scattering detection is developed for the determination of residual octylglucoside (OG) levels after a detergent exchange step for in-process samples of a vaccine antigen. The reversed-phase column not only provides separation of the OG but also functions as an extraction column to remove the vaccine antigen ...

A new method for feature extraction and recognition, namely the fuzzy bidirectional weighted sum criterion (FBWSC) is proposed in this paper. FBWSC defines the row directional fuzzy image optimal image projection matrix. Subsequently, each sample in the original training sample set is transformed using the row directional optimal image projection matrix, and the row directional feature training...

Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extr...