Several carriers were evaluated for use in the delivery of exogenous glucocerebrosidase to monocytes from Gaucher's disease patients. Only gamma globulin-coated, resealed erythrocytes proved to be an effective vehicle for enzyme delivery. Glucocerebrosidase added in this manner normalized intracellular enzyme levels for at least 18 hr. In this model system for the study of enzyme replacement th...

Studies by Rotman and Spiegelman (1954) and Hogness et al. (1955) on the 3-galactosidase of Escherichia coli have revealed that virtually all of the carbon and sulfur of that enzyme is derived from exogenous sources when cells are induced during growth. These investigations have thus ruled out the direct participation of a specific preformed precursor in the formation of fl-galactosidase in gro...

The cytochrome P450 enzyme superfamily (CYP), E.C. 1.14.-.-, are haem-containing monooxygenases with a vast range of both endogenous and exogenous substrates. These include sterols, fatty acids, eicosanoids, fat-soluble vitamins, hormones, pesticides carcinogens as well drugs. Listed below the human enzymes, their relationship rodent CYP activities is obscure in that species orthologue may not ...

The relationship between hydrogen peroxide and polyamine metabolism the role of NADPH oxidase (NOX) in this under drought conditions has not yet been clarified. To reveal relationship, expression levels genes metabolism, such as arginine decarboxylase (ADC), agmatine aminohydrolase (AIH), spermidine synthase (SPDS), S-adenosyl methionine (SAMDC), diamine (DAO) (PAO) were determined by RT PCR st...

A convenient and rapid method for the purification of Streptococcus mutans dextransucrase is described. Affinity chromatography, on a column containing insoluble dextran purified from a culture of S. mutans 6715-49, gave an almost 300-fold purification, with 76% recovery of enzyme. Subsequent hydrophobic chromatography on butyl-agarose increased the overall enzyme purification to more than 1,00...

Assessments of an Exogenous Proteolytic Enzyme in Beef Steer Diets to Improve Growth Performance and Ruminal fermentation