In conventional microscopes, fluorescence emission is separated from the backscattered illumination using the Stokes shift, whereby the emission occurs at a longer wavelength to the excitation. Such separation is usually achieved through a combination of wavelength filters that divide the spectrum into mutually exclusive excitation and emission bands. It is therefore impossible in these microsc...

OBJECTIVE At 380 nm excitation, cervical tissue fluorescence spectra demonstrate characteristic changes with both patient age and the presence of dysplasia. A Monte Carlo model was developed in order to quantitatively examine how intrinsic NADH and collagen fluorescence, in combination with tissue scattering and absorption properties, yield measured tissue spectra. METHODS Excitation-emission...

The dataset presented in this study encompasses fluorescence excitation–emission matrices (EEMs) and UV-spectroscopy data of 24 extra virgin olive oils (EVOOs) commercially available at supermarkets Switzerland. To investigate the effect thermal degradation, samples were exposed to accelerated ageing 60 ∘C up 53 days. EEMs UV absorption parameters measured 10 steps. can be used, for example, pr...

Extensive collagen cross-linking affects the mechanical competence of articular cartilage: it can make the cartilage stiffer and more brittle. The concentrations of the best known cross-links, pyridinoline and pentosidine, can be accurately determined by destructive high-performance liquid chromatography (HPLC). We explore a nondestructive evaluation of cross-linking by using the intrinsic fluo...

OBJECTIVE The objective of this study was to compare the effects of chemopreventive agents on natural fluorescence emission of ovarian cells in a cell culture and in a primate model as a feasibility trial to monitor drug activity. METHODS Fluorescence emission spectra were collected from normal (NOE) and immortalized ovarian surface epithelial cells at 290, 360, and 450 nm excitation. Redox p...

A three-way resolution method based on PARAFAC analysis of fluorescence excitation/emission matrices (EEMs) is presented to study the black kinetic system of simultaneous degradations of chlorophyll a and b extracted with other interferents from fresh spinach. The excitation and emission spectral profiles as well as the kinetic concentration profiles of chlorophyll a, b and their degradation pr...

In this paper, the fluorescence behaviour of nano colloids of ZnO has been studied as a function of the excitation wavelength. We have found that excitation at the tail of the absorption band gives rise to an emission that shifts with the change of the excitation wavelength. The excitation wavelength dependent shift of the fluorescence maximum is measured to be between 60 and 100 nm. This kind ...

Fluorescence spectroscopy has been demonstrated to be a powerful tool for characterizing phytoplankton communities in marine environments. Using different fluorescence spectra techniques, it is now possible to discriminate the major phytoplankton groups. However, most of the current techniques are based on fluorescence excitation measurements, which require stimulation at different wavelengths ...

A novel and simple method to prepare well dispersed single-walled carbon nanotubes with strong visible fluorescence in water is reported. The visible fluorescence was found to be responsive to pH value and metal ions, and tunable emission ability of oxidized SWCNTs depending on the excitation wavelength and a novel self-excitation and emission process were found.