We report the rescue of a defective vaccinia virus, forming the basis for a stringent selection protocol to generate replicating recombinant virus without the need for marker cassettes and selection agents. Plaques of recombinant virus could be isolated solely by their ability to grow in wild-type cells normally supporting the growth of vaccinia virus. All growth-competent clones analyzed conta...

Tropical and Emerging Infectious Diseases Division, Menzies School of Health Research, Charles Darwin University, and Northern Territory Clinical School, Flinders University, Darwin, NT. Bart J Currie, FRACP, DTM+H, Professor in Medicine. Reprints will not be available from the author. Correspondence: Professor B J Currie, Menzies School of Health Research, PO Box 41096, Casuarina, NT 0811. bar...

A dot-blot immunoassay for the detection of vitellogenin (Vtg) in plasma of adult grey mullet (Mugil cephalus) was developed. The assay identified the sex of the tested fish prior to detectable gonadal development, enabling the establishment of broodstock at the desired ratio of 7:4 females to males. This broodstock was maintained under natural photoperiod, and used to study the relative effect...

The topic of evacuation analysis plays an important role in the maritime field, because its natural link to safety and also relevant SOLAS requirements. In this context, paper focuses on description, testing validation agent-based mathematical model. As primary goal, model has been developed targeting a use simulations using immersive virtual reality, with possibility real-time human participat...

The administration of drugs directly into the central nervous system using polymers as drug carriers may improve the treatment of malignant brain tumors. In this study, the effect of the interstitial, localized delivery of 1,3-bis(2-chloroethyl)-l-nitrosourea (BCNU) incorporated into controlled release polymers implanted adjacent to the 9L gliosarcoma was assessed in s.c. and intracranial (i.c....

The administration of drugs directly into the central nervous system using polymers as drug carriers may improve the treatment of malignant brain tumors. In this study, the effect of the interstitial, localized delivery of 1,3-bis(2-chloroethyl)-l-nitrosourea (BCNU) incorporated into controlled release polymers implanted adjacent to the 9L gliosarcoma was assessed in s.c. and intracranial (i.c....

Background and Objectives: To compare the efficacy of three surgical methods in tonsillectomy patients; combination monopolar bipolar diathermy (diathermy), coblation dissection using Evac (coblation), Harmonic scalpel assisted (harmonic). Materials Methods: Ninety-seven patients underwent a single institute between January 2020 May 2021. Postoperative pain was evaluated visual analogue scale (...

The administration of drugs directly into the central nervous system using polymers as drug carriers may improve the treatment of malignant brain tumors. In this study, the effect of the interstitial, localized delivery of 1,3-bis(2-chloroethyl)-l-nitrosourea (BCNU) incorporated into controlled release polymers implanted adjacent to the 9L gliosarcoma was assessed in s.c. and intracranial (i.c....