Cultured dissociated cells from rat embryo cerebral hemisphere iqcorporate [gH]and [U-14C]ethanolamine into cellular lipids. Nearly all radioactivity in the lipid fractions is incorporated into 1,2diacylethanolamine phosphoglycerides and I-alkenyl,P-acylethanolamine phosphoglycerides (plasmalogen). Kinetic data suggest that the rate of labeling of both ethanolamine phospholipids from the phosph...

A method for the separation and quantitative estimation of ethanolamine, dimethyl ethanolamine, and monomethyl ethanolamine derived from lipids is described. After acid hydrolysis of the lipids, the bases are extracted from the neutralized hydrolysate with t-butonol and separated by gas-liquid chromatography.

Among the more commonly recognized constituents of naturally occurring phospholipides are phosphatidyl ethanolamine and phosphatidyl serine. The most feasible analytical procedures for these components have been based on the determination of ethanolamine and serine in a hydrolysate of the lipide. In general, assays for serine and ethanolamine have consisted of three main steps: (a) hydrolysis o...

The role of serine as a precursor and metabolic regulator for phosphatidylethanolamine biosynthesis in the hamster heart was investigated. Hearts were perfused with 60 p~ [l-SH]ethanolamine in the presence or absence of serine for up to 60 min. Ethanolamine uptake was attenuated by 0.05-10 mM serine in a noncompetitive manner, and the incorporation of labeled ethanolamine into phosphatidylet...

The effects of eritadenine, a constituent of the Lentinus edodes mushroom, and ethanolamine, the base constituent of phosphatidylethanolamine (PE), on fatty acid desaturase activities and lipid profiles were investigated comparatively in rats. Rats were fed a control diet or a diet supplemented with either eritadenine (0.05 g/kg) or ethanolamine (8 g/kg) for 14 d. Eritadenine and ethanolamine h...

A base exchange reaction for synthesis of phosphatidylethanolamine by the endoplasmic reticulum of castor bean (Ricinus comminus L. var Hale) endosperm has been examined. The calculated Michaelis-Menten constant of the enzyme for ethanolamine was 5 micromolar and the optimal pH was 7.8 in the presence of 2 millimolar CaCl(2). l-Serine, N-methylethanolamine and N,N-dimethylethanolamine all reduc...

In barley, glycine betaine is a metabolic end product accumulated by wilted leaves; betaine accumulation involves acceleration of de novo synthesis from serine, via ethanolamine, N-methylethanolamines, choline, and betaine aldehyde (Hanson, Scott 1980 Plant Physiol 66: 342-348). Because in animals and microorganisms the N-methylation of ethanolamine involves phosphatide intermediates, and becau...

Conversion of ethanolamine to choline (l), with methionine serving as the methyl donor (2), has been established in the intact animal. Stetten (1) observed that the labeled nitrogen of ethanolamine-N15 was retained in the choline molecule, and we determined that the carbon chain of ethanolamine is the precursor in vivo of the choline carbon chain (3). Conversion of ethanolamine to choline by in...

2-Aminoethylarsonic acid was tested for its ability to act as a substrate for ethanolamine-phosphate cytidylytransferase as a cytidylyl acceptor in place of ethanolamine phosphate. The expected product, like all mixed anhydrides of arsonic acids, should hydrolyse spontaneously with regeneration of the substrate analogue and CMP formation; such CMP production was observed. The limiting velocity ...

Ethanolamine phosphotransferase (EPT) is a key enzyme responsible for the synthesis of ethanolamine glycerophospholipids. Plasmenylethanolamine is a predominant molecular subclass of ethanolamine glycerophospholipids in the heart. The present study was designed to identify the selective use of 1-O-alk-1'-enyl-2-acyl-sn-glycerol as a substrate for EPT as a mechanism responsible for the predomina...