نتایج جستجو برای: est ssr markers

تعداد نتایج: 251106  

Journal: :Genetics and molecular research : GMR 2015
R Zhou Z Wu X Cao F L Jiang

In the current study, morphological traits and molecular markers were used to assess the genetic diversity of 29 cultivated tomatoes, 14 wild tomatoes and seven introgression lines. The three components of the principal component analysis (PCA) explained 78.54% of the total morphological variation in the 50 tomato genotypes assessed. Based on these morphological traits, a three-dimensional PCA ...

2007
Rajeev K. Varshney Kamel Chabane Prasad S. Hendre Ramesh K. Aggarwal Andreas Graner

A set of 16 expressed sequence tag (EST)-derived simple sequence repeat (SSR) and 15 EST-derived single nucleotide polymorphism (SNP) markers together with 4 amplified fragment length polymorphism (AFLP) primer combinations were analyzed on 43 wild (Hordeum vulgare ssp. spontaneum – HS), 35 cultivated (H. vulgare ssp. vulgare – HV) and 12 elite (H. vulgare ssp. vulgare – from EU) barley lines. ...

Journal: :The Journal of heredity 2011
Dilpreet S Riar Sachin Rustgi Ian C Burke Kulvinder S Gill Joseph P Yenish

Prickly lettuce (Lactuca serriola L.) is a problematic weed of Pacific Northwest and recently developed resistance to the auxinic herbicide 2,4-D. There are no publically available simple sequence repeat (SSR) markers to tag 2,4-D resistance genes in L. serriola. Therefore, a study was conducted to develop SSR markers from expressed sequence tags (ESTs) of 5 Lactuca species. A total of 15,970 S...

Journal: :Genome 2004
Ju-Kyung Yu Trevor M Dake Sukhwinder Singh David Benscher Wanlong Li Bikram Gill Mark E Sorrells

Expressed sequence tags (ESTs) are a valuable source of molecular markers. To enhance the resolution of an existing linkage map and to identify putative functional polymorphic gene loci in hexaploid wheat (Triticum aestivum L.), over 260,000 ESTs from 5 different grass species were analyzed and 5418 SSR-containing sequences were identified. Using sequence similarity analysis, 156 cross-species ...

Journal: :Bulletin of entomological research 2012
S Jing B Liu L Peng X Peng L Zhu Q Fu G He

To assess genetic diversity in populations of the brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae), we have developed and applied microsatellite, or simple sequence repeat (SSR), markers from expressed sequence tags (ESTs). We found that the brown planthopper clusters of ESTs were rich in SSRs with unique frequencies and distributions of SSR motifs. Three hundred and fifty-o...

2013
Qiang Fan Sufang Chen Mingwan Li Shiyang He Renchao Zhou Wenbo Liao

UNLABELLED PREMISE OF THE STUDY Firmiana consists of 12-16 species, many of which are narrow endemics. Expressed sequence tag (EST)-simple sequence repeat (SSR) markers were developed and characterized for size polymorphism in four Firmiana species. • METHODS AND RESULTS A total of 102 EST-SSR primer pairs were designed based on the transcriptome sequences of F. danxiaensis; these were the...

2015
Yuejin Zhang Yuanyuan Chen Ruihong Wang Ailin Zeng Michael K. Deyholos Jia Shu Hongbo Guo

A large scale of EST sequences of Polyporales was screened in this investigation in order to identify EST-SSR markers for various applications. The distribution of EST sequences and SSRs in five families of Polyporales was analyzed, respectively. Mononucleotide was the most abundant type, followed by trinucleotide. Among five families, Ganodermataceae occupied the most SSR markers, followed by ...

Journal: :Genetics and molecular research : GMR 2011
R F Missio E T Caixeta E M Zambolim G F Pena L Zambolim L A S Dias N S Sakiyama

Coffee is one of the main agrifood commodities traded worldwide. In 2009, coffee accounted for 6.1% of the value of Brazilian agricultural production, generating a revenue of US$6 billion. Despite the importance of coffee production in Brazil, it is supported by a narrow genetic base, with few accessions. Molecular differentiation and diversity of a coffee breeding program were assessed wi...

Journal: :Genetics and molecular research : GMR 2012
M R Bakhtiarizadeh B Arefnejad E Ebrahimie M Ebrahimi

Many years of domestication and breeding have given rise to the wide range of chicken breeds that exist today; however, an increasing number of local chicken breeds are under threat of extinction. A comprehensive characterization of chicken markers (especially type I markers) is needed to monitor and conserve genetic diversity in this species. The explosion of genomics and functional genom...

2017
Jingfang Chen Ronghua Li Yanshi Xia Guihua Bai Peiguo Guo Zhiliang Wang Hua Zhang Kadambot H M Siddique

Flowering Chinese cabbage is one of the most important vegetable crops in southern China. Genetic improvement of various agronomic traits in this crop is underway to meet high market demand in the region, but the progress is hampered by limited number of molecular markers available in this crop. This study aimed to develop EST-SSR markers from transcriptome sequences generated by next-generatio...

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