نتایج جستجو برای: est ssr
تعداد نتایج: 32398 فیلتر نتایج به سال:
White clover (Trifolium repens L.) is an allotetraploid species (2n = 4X = 32) that is widely distributed in temperate regions and cultivated as a forage legume. In this study, we developed expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers, constructed linkage maps, and performed comparative mapping with other legume species. A total of 7982 ESTs that could be assembled ...
The common vetch (Vicia sativa subsp. sativa), a self-pollinating and diploid species, is one of the most important annual legumes in the world due to its short growth period, high nutritional value, and multiple usages as hay, grain, silage, and green manure. The available simple sequence repeat (SSR) markers for common vetch, however, are insufficient to meet the developing demand for genetic...
برآورد سطح تنوع ژنتیکی درون و بین جمعیت های جو گامی ضروری جهت نگه داری منابع ژنتیکی و توسعه استراتژی-های آتی جهت اصلاح آن محسوب می شود. در پژوهش حاضر تنوع و روابط ژنتیکی 119 ژنوتیپ بومی و 25 لاین پیشرفته و تجاری با استفاده از نشانگرهای ssr و est-ssr بررسی شد. از 38 جفت آغازگر ssr مورد استفاده، 32 آغازگر چندشکلی نشان داد
UNLABELLED With the advent of high-throughput sequencing technology, sequences from many genomes are being deposited to public databases at a brisk rate. Open access to large amount of expressed sequence tag (EST) data in the public databases has provided a powerful platform for simple sequence repeat (SSR) development in species where sequence information is not available. SSRs are markers of ...
Microsatellite or simple sequence repeat (SSR) is one of the most widely distributed molecular markers that have been widely utilized to assess genetic diversity and genetic mapping for important traits in plants. However, the understanding of microsatellite characteristics in Arachis species and the currently available amount of high-quality SSR markers remain limited. In this study, we identi...
Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potential SSRs with a minimum of five repetiti...
Microsatellites or simple sequence repeats (SSRs) are highly useful molecular markers for plant improvement. Expressed sequence tag (EST)-SSR markers have a higher rate of transferability across species than genomic SSR markers and are thus well suited for application in cross-species phylogenetic studies. Our objectives were to examine the amplification of tall fescue EST-SSR markers in 12 gra...
Two methods were used to develop pineapple microsatellite markers. Genomic library-based SSR development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 Simple Sequence Repeat (SSR) loci were dinucleotide repeats (39 AC/GT repeats and 52 GA/TC repeats, accounting for 42.9% and 57.1%, resp.), and the other three ...
The adzuki bean (Vigna angularis (Ohwi) Ohwi and Ohashi) is an important grain legume of Asia. It is cultivated mainly in China, Japan and Korea. Despite its importance, few genomic resources are available for molecular genetic research of adzuki bean. In this study, we developed EST-SSR markers for the adzuki bean through next-generation sequencing. More than 112 million high-quality cDNA sequ...
تعیین سطح تنوع ژنتیکی و روابط بین ژنوتیپ ها اساس شناسایی والدین مناسب برای اهداف اصلاحی مختلف بشمار می رود. در این راستا نشانگرهای مولکولی بطور موفقیت آمیز در ارزیابی تنوع ژنتیکی انواع گیاهان زراعی مورد استفاده قرار گرفته اند. در این آزمایش، تنوع ژنتیکی 35 لاین و رقم جو، با استفاده از 19 جفت آغازگر ssr و est-ssr بررسی شد که در مجموع 157 آلل چند شکل با میانگین 85/7 آلل به ازای هر جفت آغازگر تکثی...
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