Clinical management of diabetes must overcome the challenge of in vivo glucose sensors exhibiting lifetimes of only a few days. Limited sensor life originates from compromised enzyme stability of the sensing enzyme. Sensing enzymes degrade in the presence of low molecular weight materials (LMWM) and hydrogen peroxide in vivo. Sensing enzymes could be made to withstand these degradative effects ...

Amine oxidase (EC 1.4.3.6) was purified from pea (Pisum sativum) seedlings with the aim of characterizing its catalytic properties. The specific activity and selectivity of the enzyme were studied with an oxygen sensor by following the kinetics of the amine oxidation reaction, catalysed by the enzyme. The enzyme catalytic constants were calculated from the transient signal of the oxygen sensor ...

This paper represents an experimental analysis of crosssensitivity, which is the main problem that usually faces one of the most important glucose sensors which is the amperometric enzyme electrode. The elimination of cross-sensitivity is based-on experimental determination of correction factors. Moreover, the solution for biocompatibility problem is discussed. Then, these solutions were electr...

An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme reductase, also known as butane‐2,3‐diol dehydrogenase ( Bacillus clausii DSM 8716 T ), is applied for detection in beer, red wine, and fermentation broth samples first time. The EIS sensor consists of an Al/p‐Si/SiO 2 /Ta O 5 layer immobilized reductase top Ta transducer by m...

The glucose-phosphorylating enzyme glucokinase has structural, kinetic, and molecular genetic features that are ideal for its primary role as glucose sensor in a network of neuro/endocrine sentinel cells that maintain glucose homeostasis in many vertebrates including humans. The glucokinase-containing, insulin-producing beta-cells of the pancreas take the prominent lead in this network, functio...