Cheese analogue is a substitute for milk cheese, which is similar in composition, appearance, characteristics and even in its intended use. In cheese analogues, the milk protein and milk fat are partly or wholly replaced by vegetable proteins (i.e. peanut protein, soybean protein) and vegetable fats and oils (i.e. partly hydrogenated vegetable fat like soybean, palm, etc). Cheese analogue are f...

This study aims to identify yeasts from spontaneously fermented herbs used for Van herby cheese and evaluate their enzymatic activities. Sequencing of partial 26S rRNA gene revealed the species Pichia membranifaciens (n = 8), Kazachstania servazzii 6), Debaryomyces hansenii 2), Kluyveromyces marxianus fermentans 2). Enzyme profiles were determined using API-ZYM strips. The isolates had diverse ...

Enzymes are biological catalysts that facilitate the conversion of substrates into products by providing favorable conditions that lower the activation energy of the reaction. An enzyme may be a protein or a glycoprotein and consists of at least one polypeptide moiety. Proteases are the most important industrial enzymes and comprise about 25% of commercial enzymes in the world. Two third of the...

The transfer of radiocesium during all phases of kefalograviera cheese making was monitored, and the corresponding transfer coefficients were determined. To reduce the radiocesium concentration of the final product, modifications of the standard cheese making procedure, as single and double curd washing, were investigated. The transfer coefficients from milk to cheese were determined as .33 and...

A cystathionine (gamma)-lyase (EC 4.4.1.1) ((gamma)-CTL) was purified to homogeneity from a crude cell extract of Lactococcus lactis subsp. cremoris SK11 by a procedure including anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration chromatography. The activity of SK11 (gamma)-CTL is pyridoxal-5(prm1)-phosphate dependent, and the enzyme catalyzes the (alpha),...

Metabolism of aromatic amino acids by lactic acid bacteria is an important source of off-flavor compounds in Cheddar cheese. Previous work has shown that alpha-keto acids produced from Trp, Tyr, and Phe by aminotransferase enzymes are chemically labile and may degrade spontaneously into a variety of off-flavor compounds. However, dairy lactobacilli can convert unstable alpha-keto acids to more-...

In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotransferase, AraT. In the present study, we purified and studied the second enzyme...

Biogenic amines (BA) are nitrogenous compounds that possess biological activity. The source of production is the microbial decarboxylation of amino acids. This compounds are found in various types of cheese. The aim of this work was to evaluate the BA content of some traditional cheeses in West Azerbaijan province Iran. For this purpose, 70 samples of Koopeh, 10 samples of Lighvan and 5 samples...