Three hundred fifty five pregnant women were tested for IgM and IgG type of antibodies by Enzyme Immuno Assay (EIA). Sero-positivity for IgM and IgG antibodies were 13% and 29% respectively. Of 212 pregnant women with abortion, 39 (18%) and 80 (38%) were sero-positive and of 143 pregnant women with normal reproductive performance, 7 (5%) and 23 (16%) were positive for IgM and IgG respectively. ...

The potency of cellular immune responses strongly depends on T cell avidity to antigen. Yet, functional avidity measurements are rarely performed in patients, mainly due to the technical challenges of characterizing heterogeneous T cells. The mean functional T cell avidity can be determined by the IFN-γ Elispot assay, with titrated amounts of peptide. Using this assay, we developed a method rev...

Chronic allograft rejection is in part mediated by host T cells that recognize allogeneic antigens on transplanted tissue. One factor that determines the outcome of a T cell response is clonal size, while another is the effector quality. Studies of alloimmune predictors of transplant graft survival have most commonly focused on only one measure of the alloimmune response. Because differing qual...

10 JALA Febru The Elispot technique has gained much attention over the past few years. Its outstanding sensitivity and general ease of performance has made it one of the most attractive assay candidates for immunomonitoring purposes, including vaccine development and vaccine trials in various phases, diagnostics, and basic research. The demands of the market call for a simplified and automated ...

There is no reliable test to diagnose cephalosporin-induced maculopapular exanthems (MPE). This study aimed to evaluate the role of enzyme-linked immunospot assay in the diagnosis of cephalosporin-induced MPE compared with skin testing. A total of 25 patients with a history of cephalosporin-induced MPE were skin tested and the frequencies of cephalosporin-specific interferon-γ-, interleukin-5-,...

Background Lyme borreliosis (LB) is multisystem infectious disease with various clinical manifestations. In Europe, LB is caused by gram-negative spirochetal bacteria of the complex Borrelia burgdorferi sensu lato. The most important for human pathogenicity are B. garinii, B. afzelii and B.burgdorferi sensu stricto. Indirect laboratory diagnostics of LB is based on serological methods such as E...

background while hepatitis e virus (hev) mostly causes self-limited disease in general population, it is more severe in pregnant women. objectives this study aimed to investigate the seroprevalence of anti-hev igg among a population of pregnant women in west azerbaijan of . patients and methods one hundred thirty six pregnant women referred to urban health centers of urmia for pursuing pregnanc...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

background herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are common infectious agents worldwide. data on prevalence of hsv-1 and hsv-2 infections are limited in asia, especially in iran. our study aimed on determination of seroepidemiology of hsv-1 and hsv-2 infections in asymptomatic healthy students of islamic azad university of kazeroun, fars province, southwest of iran. patients ...