cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

background: retroviruses of human t-lymphotropic viruses (htlv-1 and htlv-2) have been demonstrated to be endemic in the north-eastern region of iran. this study aims to determine htlv-1 and htlv-2 prevalence among healthy individuals in neyshabur city during 2010-2014. methods: a total of 8054 blood samples were collected from healthy participants in neyshabur, north-eastern iran. the blood sa...

results 34 (34%) of the renal transplant recipients and 26 (26%) of the control group were positive for anti-toxoplasma igg. in addition 18 (18%) of the renal transplant recipients and 4 (4%) of the control group were positive for anti-toxoplasma igm. for both values of the antibodies, differences were statistically significant (p < 0.025 and p < 0.001 respectively). the pcr results indicated t...

conclusions the present study supported the contamination with t. gondii as a risk factor for schizophrenia just in women. results the analyses confirmed the significant differences between healthy women and ones with schizophrenia (p = 0.001) as well as between males and females with schizophrenia (p = 0.009) in igg positivity. materials and methods t. gondii igg antibodies of 100 patients wit...

background there are different types of aflatoxins, including aflatoxin m1, m2, g1, g2, b1 and b2 produced in the food chain, especially when the food is infected with fungi and can directly be consumed by humans. considering that our country is located in the subtropical geographic region where temperature and humidity ecological factors are among determining factors in contamination with afm1...