This study investigated the use of acid hydrolyzed algae to enhance the enzymatic hydrolysis of lignocellulosic biomass. The farm-waste grown algal samples were first characterized, and the optimal conditions for algal hydrolysis using dilute sulfuric acid were determined. Neutralized algal hydrolysate was then tested as a reaction medium (replacing the pH buffer solution) for the enzymatic hyd...

A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, w...

OBJECTIVES The aim of this work was to evaluate saliva and tongue coating pH in oral healthy patients with morning bad breath before and after use of different oral mouthrinses. MATERIAL AND METHODS Saliva and tongue coating pH of 50 patients allocated in 5 groups were measured respectively by a digital pHmeter and color pH indicators, before, immediately after and 30 min after rinsing 5 diff...

The combinations of gel electrophoresis or LC and mass spectrometry are two popular approaches for large scale protein identification. However, the throughput of both approaches is limited by the speed of the protein digestion process. Present research into fast protein enzymatic digestion has been focused mainly on known proteins, and it is unclear whether these results can be extrapolated to ...

Biodegradable, waste-derived hydrogels containing immobilised peroxidase were successfully used for enzymatic removal of organic pollutants in water. Hydrogels are reusable and provide detoxification the contaminants solution.

Fruit industries require convenient peeling method, especially during puree processing to prevent deterioration of fruit quality and product loss. Therefore, manual, chemical (sodium hydroxide/NaOH) and enzymatic (Pectinex Ultra SP-L) peeling methods were compared to determine the peeling efficiencies of ‘Chok Anan’ mangoes. The effect of different peeling parameters (concentrations [chemical p...

protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in saccharomyces cerevisiae and kluyveromyces marxianus. crude protein content of dried yeast cells were 53.22% and 45.6% for s.cerevisiae and k.marxianus, respectively. after 96 hrs of...

Three common pretreatment processes based on dilute sulfuric acid, dilute sodium hydroxide and heat treatment (autoclaving) followed by enzymatic hydrolysis were evaluated to provide comparative performance data. Among them, the best result was obtained when the pretreatment of rice husk was carried out with 3% of NaOH solution. The pretreatment of rice husk with NaOH substantially increased th...