Chitosan was depolymerized either by HCl hydrolysis or enzymatic degradation with a commercial preparation Pectinex Ultra Spl. The chitooligosaccharides released by both methods were selectively precipitated in methanol solutions and characterized using MALDI-TOF mass spectrometry. Differences between the two methods were detected and concerned the degrees of polymerization of the fragments pro...

Polycondensation in batch is the classical way to produce polyesters. However, this method requires harsh conditions reach high conversions and drive equilibrium of reaction forward. In order controlled polymers a greener way, we worked on enzymatic ring opening polymerization (e-ROP) lactones using CALB lipase immobilized porous beads (Novozym® 435) as catalyst 3-phenyl-1-propanol (PP) initiat...

[Structure: see text] The synthesis of (S)-glycerol nucleoside triphosphates (gNTPs) and the analysis of their substrate activities for enzymatic polymerization is described. NTPs with simplified carbohydrate backbones such as the tNTPs (alpha-L-threose-NTPs) are polymerase substrates and offer the potential to create non-natural aptamer sequences with simplified backbones through enzymatic mea...