In this study the bacterial growth inhibitory activity of peptide fragments produced from goat’s milk whey proteins by enzymatic hydrolysis using trypsin, ficin and a combination of both was investigated. Goat’s milk whey proteins were isolated and subjected to enzymatic hydrolysis and peptides were purified by ultrafiltration followed by reverse-phase high-performance liquid chromatography (RP...

Enzymatic hydrolysis of pre-treated wheat straw has been studied. Several additives have checked as potential improvers glucose yield in the enzymatic hydrolysis, trying to avoid cellulases inhibition by lignin a cause decrease saccharification rate lignocellulosic biomass. Non-ionic surfactants, such Tween 80 (poly(oxyethylene)20-sorbitan-monooleate) and polyethylene glycol-6000, proteins, bov...

In the course of investigations in these laboratories on the effect of steroid hormones on various enzymatic systems, it was noted that desoxycorticosterone exerted a greater inhibitory effect than other steroids, and that the n-amino acid oxidase system was particularly strongly affected. This paper deals with experiments elucidating in part the characteristics of the n-amino acid oxidasc-deso...

Free radicals can cause various kinds of degenerative diseases and skin aging. be neutralized with an enzymatic antioxidant, namely superoxide dismutase (SOD). One the plants that has high SOD activity is salam (Syzygium polyanthum). The aim this study was to determine crude extract bay leaves precipitated ammonium sulfate salt at concentrations 25, 50, 75%. began plant determination, enzyme ex...

The classical theory of enzymatic inhibition aims to quantitatively describe the effect of certain molecules—called inhibitors—on the progression of enzymatic reactions, but growing signs indicate that it must be revised to keep pace with the single-molecule revolution that is sweeping through the sciences. Here, we take the single enzyme perspective and rebuild the theory of enzymatic inhibiti...

Since the discovery of enzymes as biological catalysts, study of their enormous catalytic power and exquisite specificity has been central to biochemistry. Nevertheless, there is no universally accepted comprehensive description. Rather, numerous proposals have been presented over the past half century. The difficulty in developing a comprehensive description for the catalytic power of enzymes ...

Enzymes are biological catalysts (also known as biocatalysts) that speed up biochemical reactions in living organisms, and which can be extracted from cells and then used to catalyse a wide range of commercially important processes. This chapter covers the basic principles of enzymology, such as classification, structure, kinetics and inhibition, and also provides an overview of industrial appl...

We examined the inhibitory action of the extract of Oren-gedoku-to, a traditional herbal medicine known to act as an antioxidant, on enzymatic lipid peroxidation in rat liver microsomes. Simultaneous addition of a spray-dried preparation of Oren-gedoku-to extract (Tsumura TJ-15) inhibited enzymatic lipid peroxidation induced by reduced beta-nicotinamide adenine dinucleotide phosphate (NADPH) an...