Monoclonal antibodies directed against the a determinant of K88 pili from porcine enterotoxigenic Escherichia coli which react with all three K88 variants have been produced. These antibodies have been used for diagnosis of porcine enterotoxigenic E. coli in a direct enzyme-linked immunosorbent assay with sensitivity to 50 ng of pilus protein per ml.

Mortality and morbidity associated with Escherichia coli cause economic losses to the swine industry. Enterotoxigenic strains of E. coli affecting nursery-age pigs have become more difficult to treat because of increased antibiotic resistance, and alternatives to antibiotics are currently being sought. Our laboratory developed a defined culture of commensal bacteria of porcine gastrointestinal ...

Low-energy dipole (LED) excitations in $^{20}\mathrm{O}$ were investigated by variation after $K$ projection of $\mathrm{deformation}\phantom{\rule{0.28em}{0ex}}(\ensuremath{\beta}$)-constraint antisymmetrized molecular dynamics combined with the generator coordinate method. A low-energy $E1$ mode, which is caused surface neutron oscillation along prolate deformation was obtained as ${1}_{2}^{\...

Purpose: To study the virulence factors, serotypes of uropathogenic Escherichia coli (UPEC) and antimicrobial suspectibility pattern. Methods: A total of 200 Escherichia coli from symptomatic cases of urinary tract infections and 50 stool samples from apparently healthy individuals were included. UPEC were screened for virulence factors namely haemolysin, mannose resistant, mannose sensitive ha...

In order to obtain sufficient quantities of beta-glucuronidase for use in structural studies, the enzyme was purified from its richest known source, the female rat preputial gland, by a method similar to that of Ohtsuka and Wakabayashi (1969) (Enzymologia 12, 109). The purified enzyme has an S-o20, w of 12.5 S and a D-o20, w of 4.3 times 10- minus 7 cm-2 S-minus 1. Sedimentation diffusion and s...

Enterotoxigenic Escherichia coli (ETEC) is a water and food-borne pathogen that infects the small intestine of the human gut and causes diarrhoea. Enterotoxigenic E. coli adheres to the epithelium by means of colonization factors and secretes two enterotoxins, the heat labile toxin and/or the heat stable toxin that both deregulate ion channels and cause secretory diarrhoea. Enterotoxigenic E. c...

background: introduction: enterotoxigenic escherichia coli (etec) is the most important bacterial cause of watery  travelers' diarrhea  in  developing countries. watery diarrhea is can cause serious life-threatening dehydration. etec was caused diarrhea by the secretion of two heat-labile enterotoxins (lts) and the heat-stable enterotoxins (sts) which increase intestinal secretion. routine labo...

With oral infection of calves by an enterotoxigenic Escherichia coli strain carrying K99, F41, and FY adhesins, colostrums from cows vaccinated against either K99+F41 or FY did not provide protection, but a mixture of the two colostrums did. The association of antibodies directed against the different adhesins is more effective than antibodies directed against one adhesin alone for colostral pr...

The protective effect of a combined rotavirus/enterotoxigenic E. coli K99 vac­ cine against naturally occurring neonatal diarrhoea was evaluated in two dairy and two beef herds. Before vaccination, frequency of diarrhoea in these herds was 46% up to 60% with losses of 7% to 20%. After vaccination of pregnant cows and heifers mild diarrhoea occurred in 14% to 20% of their calves, one out of 144 ...