Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of ...

Raw oats are frequently contaminated by fungi producing mycotoxins, and as such, they can pose a health risk to humans regularly consuming oat products. To reveal the effect of particular processing steps on contamination levels in final products, two series samples obtained from an flake manufacturer were analysed. Among fractions treated, most significant mycotoxin decrease was demonstrated c...

The production of enniatins by Fusarium scirpi during fermentative growth in submerged cultures was measured. The fungus produced the antibiotic during mycelial growth, but not during the stationary phase of cultivation. By contrast, enniatin synthetase, the enzyme responsible for enniatin synthesis, was present during growth, during the stationary phase, and even in spores. Similarly, the enni...

This study developed and validated an analytical methodology for the determination of aflatoxins, enniatins, beauvericin, zearalenone, ochratoxin-A, alternariols, HT-2 T-2 toxin in soy, oat, rice almond beverages, based on solid phase extraction columns (SPE) analyzed by liquid chromatography coupled to mass spectrometry tandem. C18 SPE was successfully applied, obtaining recoveries that range ...

The efficacy of cold atmospheric pressure plasma (CAPP) with ambient air as working gas for the degradation of selected mycotoxins was studied. Deoxynivalenol, zearalenone, enniatins, fumonisin B1, and T2 toxin produced by Fusarium spp., sterigmatocystin produced by Aspergillus spp. and AAL toxin produced by Alternaria alternata were used. The kinetics of the decay of mycotoxins exposed to plas...

Durum wheat grain can accumulate mycotoxins because it is highly sensitive to infections caused by pathogens of the genera Fusarium and Alternaria. Reduced fungicide use increases demand for biological methods pathogen control. The aim experiment was evaluate efficacy Debaryomyces hansenii (Dh) yeast in reducing content secondary fungal metabolites present spikes five durum cultivars grown sout...

Enniatins are so-called “emerging mycotoxins” that commonly occur in milligrams per kilogram levels grains and their derived products, as well fish, dried fruits, nuts, spices, cocoa, coffee. The present study investigated the 28-day repeated oral dose toxicity of enniatin complex CD1(ICR) mice. Enniatin B, B1, A1 at a ratio 4:4:1 were administered to male female mice doses 0 (vehicle controls)...