KIT receptor tyrosine kinase is expressed in tumor endothelial cells of adult glioblastomas, but its expression in pediatric brain tumor endothelial cells is unknown. We assessed expression of KIT, phosphorylated KIT, stem cell factor (SCF) and vascular endothelial growth factor receptor-2 (VEGFR-2) in 35 juvenile pilocytic astrocytomas and 49 other pediatric brain tumors using immunohistochemi...

Abstract All capillary endothelia, including those of the glomeruli, have a luminal cell surface layer (ESL) consisting glycoproteins, glycolipids, proteoglycans (PGs) and glycosaminoglycans. Previous results demonstrated that an intact ESL is necessary for normal filtration barrier damage to coupled proteinuria seen example in diabetic kidney disease (DKD). We used principles ion exchange chro...

PURPOSE To investigate cell surface-associated keratan sulfate on the corneal endothelium. METHODS Immunolabeling techniques were used at the light, scanning, and transmission electron microscopic level to localize keratan sulfate on the corneal endothelium. The investigation included human, bovine, and rabbit corneal endothelia. A quantitative study of the relationship between cell size and ...

Careful study of capillary endothelial cells as part of the stroma reaction in human malignant melanoma showed branched tubular structures (BTS). These structures were found in five cases, two of which were melanoma superficial spreading type with strong host cellular response (level 2 invasion), and one of which was a nodular melanoma (level 3 invasion) associated with a good host-cellular TAB...

The pathologic study showed a proliferation of vascular structures in the dermis, mostly capillaries with prominent endothelia, together with marked stromal cell hyperplasia and the presence of multinucleated giant cells, frequent mast cells, lymphocytes, and plasma cells (Figures 2 and 3). On immunohistochemical analysis, the vascular structures were positive for CD34 and the stromal cells for...

Brain endothelium has a distinctive phenotype, including high expression of transferrin receptor, p-glycoprotein, claudin-5 and occludin. Dermal endothelium expresses lower levels of the transferrin receptor and it is absent from lung endothelium. All three endothelia were screened for transcription factors that bind the transferrin receptor promoter and show different patterns of binding betwe...