two separate factorial experiments were conducted to study the effects of explant sources, plant growth regulators, sucrose concentrations, and light conditions on in vitro cormlet formation of freesia (freesia ×hybrida bailey 'argenta'). interestingly, it was observed that the pupae had lower contamination levels compared to mother corms. using 40% sodium hypochlorite solution for 40...

salt stress negatively impacts crops yield throughout the world. nine varieties of potato (solanum tuberosum l.) were screened for salt stress tolerance by measuring in-vitro growth of the aerial plant parts, as well as roots. salt stress was evaluated by adding 25, 50, 75, 100, 125, 150 and 200 mm of nacl to murashige- skoog (ms) medium and compared to ms medium without nacl. plant length and ...

background: in this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. methods: the developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of 20 ...

Background: In vitro culture of ovarian follicles may preserve fertility in women with premature ovarian failure due to cancer .It seems that creation a condition that could maintain cellular communications and supports growth of follicles to produce mature oocytes appear to be essential. Nitric oxide (NO) has been recently shown to act with a dual action in mouse oocyte meiotic maturation depe...

STUDY QUESTION Is thymic stromal lymphopoietin (TSLP) involved in the pathophysiology of endometriosis? SUMMARY ANSWER TSLP is up-regulated by interleukin (IL)-1β and may be involved in the development of endometriosis. WHAT IS KNOWN ALREADY Endometriosis is a chronic inflammatory disease in which the Th2 immune response is activated and has been suggested to promote the disease. TSLP is a ...

OBJECTIVE Now there are more and more evidences that Cyclooxygenase-2 (COX-2) plays an important role in angiogenesis of endometriosis (EMs). Vascular endothelial growth factor (VEGF) has a potent angiogenic activity. However, it is worth studying about the regulating mechanism of COX-2/COX-1 and VEGF in the development of human endometriosis in vitro. The current study was designed to investig...

the aim of the present study was to develop an in vitro system for functional investigation of ovarian follicular cells in sterlet, acipenser ruthenus. oocytes for the primary culture were obtained from the ovaries of a 6 years old sterlet 729 g in weight and 47 cm in total length. the oocytes were in advanced vitellogenesis stage (pi >10). a part of the ovary (containing about 300 follicles) w...

scented rose species are very important in the genus rosa due to their use in highly-prized essential oil production and as garden roses. the fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. a comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly damask r...

background: endometriosis is one of the most challenging diseases that constitute 20% - 40% of women searching for their infertility diagnosis. objective: this study was undertaken in order to compare the outcome of in vitro fertilization/intracytoplasmic sperm injection (ivf/icsi) in women with endometriosis, and tubal factor infertility as controls. materials and methods: from 2005 to 2006 a ...