A truncated derivative of the phage endolysin LysK containing only the CHAP (cysteine- and histidine-dependent amidohydrolase/peptidase) domain exhibited lytic activity against live clinical staphylococcal isolates, including methicillin-resistant Staphylococcus aureus. This is the first known report of a truncated phage lysin which retains high lytic activity against live staphylococcal cells.

Title of dissertation: ENGINEERING ENHANCED STRUCTURAL STABILITY TO THE STREPTOCOCCAL BACTERIOPHAGE ENDOLYSIN PLYC Ryan Daniel Heselpoth, Doctor of Philosophy, 2014 Dissertation Directed by: Associate Professor Daniel C. Nelson Institute for Bioscience and Biotechnology Research and Department of Veterinary Medicine Antibiotic misuse and overuse has prompted bacteria to rapidly develop resistan...

Staphylococcal peptidoglycan hydrolases are a potential new source of antimicrobials. A large subset harbors C-terminal SH3b_5 cell wall binding domains. These C-terminal domains have been shown to be necessary for accurate cell wall recognition and subsequent staphylolytic activity for some endolysins. Over fifty proteins of staphylococcal or phage origin containing SH3b domains were aligned, ...

UNLABELLED An open reading frame with homology to known endolysin genes was identified in the genome of Streptomyces sp. strain 212, which is a newly isolated soil bacterium. The heterologously expressed gene product of this endolysin-like gene, called Mitrecin A, demonstrated bacteriolytic activity against several Gram-negative bacteria. The genome of the bacterial strain was sequenced to draf...

Human typhoid fever caused by Salmonella Typhi still poses a severe global disease burden in developing countries despite the availability of commercial vaccines. In this study, we constructed a non-living S. Typhi Ty21a vaccine candidate by employing a lambda (λ) phage-derived holin-endolysin system to efficiently construct bacterial ghosts. The lysis plasmid pJHL464 harbors an R lysis cassett...

in the present study, the therapeutic potential of bacteriophages virulent to staphylococcus aureus associated with goat mastitis were isolated, identified and assessed. staphylococcus aureus (host or indicator bacterium) was isolated from a goat suffering from clinical mastitis. based on cultural, morphological, biochemical tests and amplification of s. aureus specific thermonuclease gene inpc...

19 20 Bacteriophage endolysins have an interesting potential as antimicrobials. The endolysin 21 LysH5, encoded by Staphylococcus aureus phage vB_SauS-phi-IPLA88, was expressed 22 and secreted in Lactococcus lactis using the signal peptide of the bacteriocin lactococcin 23 972 and lactococcal constitutive and inducible promoters. Up to 80 U/mg of 24 extracelullar active endolysin was detected i...

The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems.

Rapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin. Title Rapid degradation of Streptococcus pyogenes biofilms by PlyC, a bacteriophage-encoded endolysin. Publication Type Journal Article Year of Publication 2013 Authors Shen, Y, Köller, T, Kreikemeyer, B, Nelson, DC Journal J Antimicrob Chemother Volume 68 Issue 8 Pagination 1818-24 Date Published 2...

BACKGROUND Rifampin-soaked synthetic prosthetic grafts have been widely used for prevention or treatment of vascular graft infections (VGIs). This in vitro study investigated the effect of the antibiotics daptomycin and vancomycin and the new recombinant bacteriophage endolysin HY-133 on vascular cells, as potential alternatives compared to rifampin. MATERIAL AND METHODS Primary human ECs, vasc...